uPA、uPAR、nm23-H1在大肠癌中的表达及其与肿瘤侵袭和转移的关系  

Expression of uPA, uPAR, nm23-H1 in Human Colorectal Cancer and Its Relation to Tumor Invasion and Metastasis

作　　者：赵茜[1] 吴秋良[2] 梁小曼[2] 区穗芳[1] 谢秀琴[1] 

机构地区：[1]广州铁路中心医院病理科,510080 [2]中山大学肿瘤医院病理科

出　　处：《肿瘤防治研究》2004年第11期694-696,F002,共4页Cancer Research on Prevention and Treatment

摘　　要：目的探讨尿激酶型纤溶酶原激活剂(uPA)、尿激酶型纤溶酶原激活剂受体(uPAR)和nm23H1基因蛋白在大肠癌中的表达及其与肿瘤侵袭和淋巴结转移的关系。方法应用免疫组化SABC法,对121例大肠癌手术根治标本进行uPA、uPAR和nm23H1基因蛋白测定。结果uPA、uPAR和nm23H1阳性表达率分别为62%、74%和48%。uPA和uPAR高表达与大肠癌侵袭和淋巴结转移关系密切(P<0.05)。nm23H1基因蛋白的低表达与大肠癌分化程度和淋巴结转移密切相关(P<0.05)。大肠癌中uPA和nm23H1蛋白表达呈负相关(P<0.05)。结论uPA、uPAR和nm23H1基因蛋白表达与大肠癌侵袭和转移有显著相关性;同时检测uPA和nm23H1表达状况,可作为预测大肠癌淋巴结转移及预后的有用指标。Objective To investigate the expression of urokinase type plasminogen activator(uPA), uPA receptor(uPAR) and nm23 H1 gene protein in human colorectal cancer and its relationship with the invasion and lymph node metastasis.Methods SABC immunohistochemical method was used to detect the uPA, uPAR and nm23 H1 gene protein expression in 121 cases with human colorectal cancer after the surgical treatment.Results The expression rate of uPA, uPAR and nm23 H1 in 121 cases were 62%, 74% and 48% respectively. A significant correlation was found between high uPA, uPAR expression and the invasion and the lymph node metastasis of colorectal cancer ( P < 0.05 ). A significant correlation was found between low expression of nm23 H1 gene protein and differentiation of the tumor and the lymph node metastasis of colorectal cancer( P < 0.05 ). In addition, there is a significant correlation between uPA and nm23 H1( P < 0.05 ).Conclusion UPA, UPAR and nm23 H1 may contribute significantly to colorectal cancer invasion and metastasis. The simultaneous detection of expression of both uPA and nm23 H1 may serve as a marker of lymph node metastasis in colorectal cancer and evaluate the prognosis of the patients with colorectal cancer.

关 键 词：大肠肿瘤 UPA UPAR NM23-H1 免疫组织化学 

分 类 号：R735.34[医药卫生—肿瘤]