闪烁光对豚鼠眼球发育及近视形成的影响  

Effects of flashing light on ocular growth and development of myopia in pigmented guinea pigs

作　　者：程振英[1] 李镜海[1] 李荣[1] 谢英勃[2] 

机构地区：[1]山东大学齐鲁医院,济南250012 [2]山东师范大学生命科学院

出　　处：《中华眼科杂志》2004年第9期601-604,共4页Chinese Journal of Ophthalmology

摘　　要：目的　探讨闪烁光对豚鼠眼球发育及近视形成的影响。方法　将 30只 4周龄豚鼠随机分为 3组 ,Ⅰ组在闪烁光照明环境中饲养 ,闪烁方式为亮 2s,暗 2s;Ⅱ、Ⅲ组在日常照明环境中饲养 ,作为对照 ,Ⅱ组照明时间为每天 12h ,Ⅲ组照明时间为每天 2 4h。 3组中光照度均为 2 0 0流明。实验 6周后分别用检影法测量眼屈光度 ,A超测量眼轴长度 ;摘除眼球 ,用电子天平测量眼球重量 ,在光学显微镜和透射电镜下观察眼球后极部巩膜、脉络膜和视网膜的组织学改变。结果　与对照组相比 ,在闪烁光照明环境中饲养的动物发生 - 7 0 0D的近视 ,眼轴增长 0 5 6mm ,眼球加重 6 8mg ,后极部巩膜细胞数量增多、活性增强、胶原纤维间隙扩大 ,脉络膜层变窄 ,视网膜感光细胞外节盘变短、排列不规则。结论　闪烁光能促进豚鼠眼球增长、诱导近视的形成。Objective To investigate the effects of flashing light exposure on ocular growt h and development of myopia in guinea pigs. Methods Thirty 4-week-old pigmented guinea pigs were randomly divided into three group s. Animals in group Ⅰ were reared with the flashing light continuously and las ted for 6 weeks. The flashing frequency was 15 times per minutes and every flas h includes 2 seconds light on and 2 seconds dark. Animals in group Ⅱand Ⅲ wer e reared with normal light on, and the illumination cycle was 12 hours light/ 1 2 hours dark in group Ⅱand 24 hours light in group Ⅲ. After 6 weeks, the eff ects of flashing light on eye development were assessed by cycloplegic retinosco py, a-scan ultrasonography and eye weight. The histopathology changes of scler a, choroids and retina in posterior pore of the eye were examined using light mi croscope and transmission electron microscope. Results At the end of the treatment period, guinea pigs reared in flashing light exhibit ed -7.00 D myopia, eye axial elongated 0.56 μm, and the weight increased 68 m g. The histopathology examination showed that the posterior sclera fibroblast b ecome more active,the cell number increased, and the place between fibers becam e larger, the posterior choroids became thinned, and the outer membrane of photo receptor cells became shorter and irregular, when compared with the control grou ps.Conclusion Flashing light can promote ocular growth a nd induce myopia in pigmented guinea pigs.

关 键 词：眼球发育 豚鼠 近视 巩膜 脉络膜 视网膜 对照组 结论 排列 照明环境 

分 类 号：R778.11[医药卫生—眼科] R779[医药卫生—临床医学]