DHPLC检测胃癌微卫星不稳定性  

DHPLC Analysis of Microsatellite Instability in Gastric Cancer

作　　者：吕炳建[1] 来茂德[2] 程蕾[2] 张宇伟[2] 

机构地区：[1]浙江大学医学院附属邵逸夫医院病理科,杭州310016 [2]浙江大学医学院病理学与病理生理学系,杭州310031

出　　处：《遗传》2004年第5期574-578,共5页Hereditas（Beijing)

基　　金：浙江省科委重大项目资助~~

摘　　要：为探讨一种快速、简便、可靠的胃癌微卫星不稳定性(MSI)检测方法,用变性聚丙烯酰胺凝胶电泳 银染法检测28例胃癌12个微卫星位点(D1S548、D1S552、D5S346、TP53、IGFⅡR(G)8、IGFⅡR(CT)5、TGFβRⅡ(GT)3、TGFβRⅡ(A)10、hMSH3(A)8、hMSH6(G)8、BAX(G)8和Bat26),DHPLC柱温50℃检测Bat26位点。凝胶电泳发现MSI H2例(7 14%),MSI L胃癌15例(53 6%),Bat26+2例均为MSI H,Bat26改变和MSI H表型一致(P<0 01,Fisher's确切概率法)。DHPLC亦证实2例Bat26+胃癌,结果和凝胶电泳完全一致。结果表明,DHPLC检测Bat26位点是研究胃癌MSI H的较好方法,有一定的临床应用价值。To establish a fast,simple and solid method of studying microsatellite instability (MSI) in gastric cancer,a panel of 12 microsatellite sites,D1S548,D1S552,D5S346,TP53,IGFⅡR(G)_8,IGFⅡR(CT)_5,TGFβRⅡ(GT)_3,TGFβRⅡ(A)_(10),hMSH3(A)_8,hMSH6(G)_8,BAX(G)_8 and Bat26,were detected by denatured polyacrymide gel electrophoresis-silver stain in 28 gastric cancers.Bat26 was also analyzed by denatured high performance liquid chromatograph (DHPLC) at 50℃ in the DNASep Cartridge.Two MSI-H (7.14%) and 15 MSI-L cancers (53.6%) were identified in 28 gastric cancers.Bat26 was positive only in 2 MSI-H cancers.The alterations of Bat26 and MSI-H status were coincident (P<0.01).The two Bat26+ cancers were also confirmed by DHPLC.Results obtained from DHPLC and gel electrophoresis were completely consistent.Thus,DHPLC analysis of Bat26 site may be a favorable method of detecting MSI-H status in gastric cancer,and be of clinical importance.

关 键 词：微卫星不稳定性 Bat26 DHPLC 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]