南京汉族群体肺癌易感性相关基因的研究  

Studies of the Genes Related to Lung Cancer Susceptibility in Nanjing Han Population, China

作　　者：梁戈玉[1] 浦跃朴[1] 尹立红[1] 

机构地区：[1]东南大学公共卫生学院劳动卫生与环境卫生学系,南京210009

出　　处：《遗传》2004年第5期584-588,共5页Hereditas（Beijing)

基　　金：国家自然科学基金(30170791);江苏省社会发展基金(BS2000034)资助项目~~

摘　　要：为了探讨南京汉族群体肺癌易感性相关基因,我们采用1:1病例对照研究方法,以PCR—RFLP技术检测了152对肺癌和健康对照的CYP1A1、CYP2E1、GSTM1、GSTT1、GSTP1、mEH和NQO1基因的基因型并分析其与肺癌的相关性。结果发现携带CYP1A1突变基因型(wt/mt和mt/mt)的个体明显增加患肺鳞癌的风险(OR=2 31,95%CI=1.23 4.36);GSTT1(-)基因型可使肺癌发生的风险增加2.06倍(95%CI=1.30 3.24);具有NQO1wt/mt与mt/mt基因型者发生肺癌的风险也有所增高(OR=1.66,95CI%=1.01 2.74);CYP1A1突变基因型与GSTT1缺失基因型、CYP1A1突变基因型与NQO1突变基因型对肺癌的发生存在协同作用,同时具有两种易感基因型的个体更容易发生肺癌。研究结果表明,CYP1A1、GSTT1、NQO1基因可能与南京汉族群体肺癌遗传易感性有关,基因型之间的联合检测更有助于高危人群的筛选。To investigate the genes related to lung cancer susceptibility in Nanjing Han population, China, a 1:1 matched case-control study was performed in which 152 hospital controls were matched to the 152 original lung cancer cases. The polymorphisms of CYP1A1, CYP2E1, GSTM1, GSTT1, GSTP1, mEH and NQO1 genes were analyzed by PCR—RFLP assay. The results showed that the heterozygote and mutation homozygote genotypes of CYP1A1 were related to the risk of squamous cell carcinoma (OR=2.31, 95%CI=1.23-4.36). The risk of suffering from lung cancer was increased 2.06-fold in the individuals with GSTT1(-) genotype (95%CI= 1.30-3.24). The genotype of NQO1 wt/mt and mt/mt was found also to be associated with the risk of lung cancer (OR=1.66,95%CI=1.01-2.74). It was shown that there was no difference in the genotype distribution of CYP2E1, GSTM1, GSTP1 or mEH between cases and controls. Furthermore, stratified analysis suggested that the combination of genotypes of both CYP1A1 and GSTT1 enzymes had a synergistic action in risk of lung cancer (OR=3.41, 95%CI =1.77-6.55). Similarly, there was a cooperation between CYP1A1 mutation genotype and NQO1 mutation genotype (OR=2.45, 95%CI=1.13-5.31). This study suggested that CYP1A1, GSTT1 and NQO1 gene polymorphisms might be associated with the susceptibility to lung cancer in Nanjing Han population. Analysis of gene-gene interactions was helpful to identification of susceptible individuals and screening high-risk population to lung cancer.

关 键 词：肺癌 代谢酶基因 多态性 遗传易感性 

分 类 号：R734.2[医药卫生—肿瘤]