卵巢上皮性癌组织中肿瘤浸润性树突状细胞的状态及其与血管内皮生长因子表达的相关性  

Tumor-infiltrating dendritic cells in epithelial ovarian carcinoma and correlation with the expression of vascular endothelial growth factor

作　　者：毛愉燕[1] 陈怀增[1] 谢幸[1] 叶大风[1] 吕卫国[1] 

机构地区：[1]浙江大学医学院附属妇产科医院妇科,杭州310006

出　　处：《中华妇产科杂志》2004年第10期693-697,i001,共6页Chinese Journal of Obstetrics and Gynecology

基　　金：浙江省2000年自然科学基金资助项目(300468)

摘　　要：目的研究卵巢上皮性癌组织中肿瘤浸润性树突状细胞(TIDC)的状态及其与血管内皮生长因子(VEGF)表达的关系。方法采用免疫组化链霉菌抗生物素蛋白过氧化酶连接法和Picture二步法,检测57例卵巢上皮性癌(恶性组)、30例良性卵巢上皮性肿瘤(良性组)及16例正常卵巢(正常组)组织中S100蛋白、CD83标记的TIDC状态及其VEGF的表达。结果(1)恶性组TIDC光镜下形态基本可分成两种,其分布有异质性。恶性组S100+TIDC中位数为43个/400高倍视野(HPF),分别与良性组(中位数为18个/HPF)和正常组(中位数为20个/HPF)比较,差异有显著性(P值分别为0000和0015)。恶性组中,早期(Ⅰ～Ⅱ期)肿瘤组织中S100+TIDC数量明显多于晚期(Ⅲ～Ⅳ期,中位数分别为60个和38个/HPF,P=0026)。恶性组中,CD+83TIDC浸润肿瘤间质者极少,中位数为0个/HPF。(2)恶性组细胞中VEGF高表达(50分),分别与良性组(38分)和正常组(24分)比较,差异均有极显著性(P=0000)。(3)恶性组中,S100+TIDC数量与VEGF表达呈明显负相关(P=0001)。结论(1)卵巢上皮性癌细胞可刺激TIDC的募集,但同时受VEGF的抑制。(2)卵巢上皮性癌中TIDC成熟严重受抑。Objective To investigate the density and activation status of tumor infiltrating dendritic cells (TIDC) in epithelial ovarian carcinoma (EOC) and correlation with the expression of vascular endothelial growth factor (VEGF) Methods Streptavidin peroxidase (SP) and Picture two step immunohistochemistry methods were used to detect S 100 +, CD + 83 TIDC and the expression of VEGF in 57 primary EOCs, 32 benign ovarian tumors (benign control) and 16 normal ovarian tissues (normal control) Results (1)Two types of heterogeneous distribution pattern of TIDC in EOC were observed under the microscope The number of S 100 + TIDC in EOC [median 4 3 cells/high power field (HPF)], was significantly higher than that in benign controls (median 1 8 cells/HPF) and normal controls (median 2 0 cells/HPF, P =0 000 and 0 015) The number of S 100 +DC in early stage was significantly higher than that in advanced stage (median 6 0 and 3 8 cells/ HPF, P =0 026) Few CD + 83 TIDCs infiltrated tumor stromal tissue in EOC ( median 0) (2) The expression of VEGF was significantly higher in EOC than in controls ( P =0 000) (3) The number of S 100 + DC in EOC was negatively correlated to the expression of VEGF in tumor cells ( P =0 001) Conclusions (1)The number of S 100 + TIDC increases significantly in EOC Ovarian carcinoma cells may stimulate recruitment of TIDC in EOC, but TIDC can be suppressed by VEGF (2) Maturation of TIDC in EOC is severely inhibited

关 键 词：恶性 卵巢上皮性癌 血管内皮生长因子 良性 癌组织中 正常 表达 链霉菌 细胞 负相关 

分 类 号：R737.31[医药卫生—肿瘤]