大鼠肢体缺血/再灌注后肝脏HO-1表达的变化及其意义  

CHANGES OF HO-1 GENES EXPRESSION IN LIVER FOLLOWING ISCHEMIA/REPERFUSION OF LIMBS AND THEIR SIGNIFICANCE IN RATS

作　　者：史中立[1] 凌亦凌[2] 凌毅群[2] 姚玉霞[2] 周君琳[2] 

机构地区：[1]河北医科大学解剖教研室,河北石家庄050017 [2]河北医科大学病理生理教研室,河北石家庄050017

出　　处：《中国应用生理学杂志》2004年第4期367-370,共4页Chinese Journal of Applied Physiology

摘　　要：目的探讨肢体缺血/再灌注(I/R)致肝脏损伤时肝组织诱导型血红素氧合酶(HO1)表达的变化及其意义。方法夹闭大鼠双侧股动脉根部4h、开放2～24h,制备肢体I/R模型。RTPCR检测肝组织HO1mRNA表达的变化,免疫组化染色法观察HO1蛋白在肝内的生成与分布。对肢体I/R大鼠应用锌原卟啉抑制其体内HO1活性后,光镜观察其肝组织的病理变化。结果肢体I/R后肝组织HO1mRNA的表达水平显著高于各对照组,再灌12h表达至峰值,至再灌24h仍显著高于各对照组(P<0.01)。肢体I/R组肝组织内出现大量弥散分布的HO1阳性肝细胞,抑制HO1活性,使肢体I/R组肝组织损伤明显加重。结论肢体I/R损伤可诱导肝细胞HO1基因表达上调,所诱生的HO1对肝细胞具有保护效应。Aim: To detect the changes of inducible heme oxygenase (HO 1) expression in liver following ischemia/reperfusion(I/R)of hindlimbs and to elucidate their significance. Methods: I/R was established using the occlusion of the femoral arteries for 4h and re opening for 2-24 h in rats. The expression of HO 1 mRNA and HO 1 protein in liver tissue were detected with reverse transcription polymerase chain reaction (RT PCR) and immunohistochemical technique, respectively. The observation of pathologic changes of li ver was made following the inhibition of HO 1 by zinc protoporphyrin (ZnPP). Results: Compared with control groups, the relative expression level of HO 1 mRNA significantly increased in I/R group. There were more HO 1 positive hepatocytes in I/R group than control groups. The pathologic changes of liver tissue became more severe in I/R+ZnPP group. Conclusion: The expressions of HO 1 mRNA and protein in liver tissue are significantly upregulated, induction of HO 1 is involved in protection for hepatocytes during the I/R of hindlimbs.

关 键 词：大鼠 肝 肢体 缺血/再灌注损伤 诱导型血红素氧合酶 

分 类 号：R363.2[医药卫生—病理学]