嗜热蛋白酶生产菌Bacillus sp.DPE_7的产酶条件研究  

Conditions for production of thermophilic proteinase by Bacillus sp.DPE_7

作　　者：张云峰[1] 李有志[2] 莫天砚[3] 

机构地区：[1]淮阴师范学院生物学系,江苏淮安223001 [2]广西大学分子遗传研究所,广西南宁530005 [3]广西大学农学院,广西南宁530005

出　　处：《广西大学学报（自然科学版）》2004年第3期231-235,共5页Journal of Guangxi University（Natural Science Edition）

摘　　要：芽孢杆菌(Bacillussp.)DPE7产嗜热蛋白酶的适宜条件,较好的碳源是0.1%葡萄糖,最佳氮源为酵母粉加硫酸铵,在pH6～11范围内产酶均良好.在65℃,175r/min,pH9的产酶培养基中发酵24h,产酶量可达23.4U/mL.0.01%MnCl2,0.15%CaCl2或0.15%吐温存在时能刺激菌株产酶.Suitable conditions have been studied for production of thermophilic proteinase by Bacillus sp.DPE7,the better carbondrates is glucose,optimum nitrogen sourse is yeast powder +(NH4)2SO4. Thermophilic proteinase is higher degree from pH 6.0 to pH 10.0.The enzyme activity of fermented culture is as high as 23.4 U/mL under optimal conditions.The products of enzymes is increment by 0.01% MnCl2,0.15% CaCl2 and 0.15% tween.

关 键 词：芽孢杆菌 嗜热蛋白酶 产酶条件 

分 类 号：TQ925.2[轻工技术与工程—发酵工程]