系统性红斑狼疮病人外周血CD8^+CD28^-细胞群的检测及意义  

Expression and significance of CD8^+CD28^- cells in peripheral blood patients with systemic lupus erythematosus

作　　者：杨佳[1] 沈茜[1] 

机构地区：[1]第二军医大学长海医院实验诊断科,上海200433

出　　处：《中华风湿病学杂志》2004年第11期669-671,共3页Chinese Journal of Rheumatology

基　　金：国家"863"高技术研究发展计划基金资助项目(2002AA214091);上海市科委自然科学基金资助项目(03ZR14026)

摘　　要：目的观察系统性红斑狼疮(SLE)病人外周血CD8+CD28-细胞群比例与疾病活动程度和临床表现的关系。方法二色流式细胞术检测SLE病人(活动期25例,稳定期26例)及正常人(30名)外周血CD8+CD28-细胞群的比例,结合病人血清免疫球蛋白含量等进行分析。结果SLE活动期病人外周血CD8+CD28-细胞群的比例较正常人略有升高,而稳定期病人较正常人显著升高;同一活动期SLE病人经过治疗病情缓解后,外周血CD8+CD28-细胞较活动期升高,但差异无显著性;复发患者外周血CD8+CD28-细胞群的比例明显高于初发患者和正常人;血清免疫球蛋白(IgG、IgA和IgM)含量正常的患者外周血CD8+CD28-细胞群的比例明显高于正常人。结论SLE病人外周血CD8+CD28-T细胞群的比例的异常与疾病的病程和临床表现相关联,可能参与了疾病的致病机制。Objectives To examine the ratio of peripheral blood CD8+CD28-T cells and explore the association with diseasestage and clinical manifestations. Methods The percentage of CD8+CD28-T cell in peripheral blood from patients with systemic lupus erythematosus (SLE) (25 in active stage and 26 in stable) and 30 normal individuals was detected by two-color flowcytometry. Results The percentage of peripheral blood CD8+CD28-T cells in patients with non-active disease was significantly increased compared to normal individuals. In the individnal patients studied longitudinally, the frequency of peripheral blood CD8+CD28-T cells in nonactive disease was slightly higher than that of patients in active disease. The ratio of peripheral blood CD8+CD28-T cell in flare patients was higher than that of untreated patients and normal individuals. The percentage of peripheral blood CD8+CD28-T cell in patients with normal serum immunoglobulin level was significantly higher than that of normal individuals. Conclusions The decrease of peripheral blood CD8+CD28-T cells in patients with SLE is related to disease stage and clinical manifestation and may play a role in the pathogenesis of SLE.

关 键 词：CD28 病人 细胞群 CD8^+ 人外周血 正常人 SLE 比例 目的观 结论 

分 类 号：R593.241[医药卫生—内科学] R392[医药卫生—临床医学]