青春期糖尿病大鼠生殖腺内5α-还原酶(2型)的基因表达研究  

Gene expression of 5α-reductase (type 2) in reproductive gland of pubertal diabetic rats

作　　者：刘素嬛[1] 王忠山[1] 许宗革[1] 辛暨丽[1] 左文静[1] 

机构地区：[1]吉林大学基础医学院细胞生物教研室,吉林长春130021

出　　处：《中国病理生理杂志》2004年第9期1580-1582,共3页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.39870313)

摘　　要：目的研究青春期糖尿病大鼠生殖功能障碍的可能分子机制。方法用链脲菌素复制青春期大鼠糖尿病模型,实验分正常对照(C)组、糖尿病(D)组及糖尿病+胰岛素治疗(ID)组,以Northernblot检测睾丸、附睾、前列腺内5α-还原酶(2型)的mRNA水平。结果附睾头部,D组和ID组大鼠的2型酶mRNA水平低于C组(P<005)。结论附睾2型5α-还原酶mRNA水平低下可能使双氢睾酮生成减少,从而影响青春期大鼠生殖系统的发育与功能。AIM: To study the molecular mechanism of reproduction dysfunction in pubertal diabetic rat, the level of 5α-reductase (type 2) mRNA in testis, epididymis and prostate in diabetic rat was detected. METHODS: The gene expression of 5α-reductase (type 2) was detected by Northern blot. RESULTS: In the caput of the epididymis, the expression of 5α-reductase (type 2) mRNA of D and ID groups was less than that of C group. CONCLUSION: The decreased expression of 5α-reductase (type 2) results in the decreased production of dihydrotestosterone, which influences the development and function of reproduction system in pubertal rats. [

关 键 词：糖尿病 氧化还原酶类 生殖 

分 类 号：R363[医药卫生—病理学]