钙通道阻滞剂对缺氧右心室心肌钙调神经磷酸酶活性的调控作用  

Blockade of L-type Ca^(2+) channels suppresses activation of calcineurin and development of right ventricle cardiac hypertrophy induced by chronic hypoxia

作　　者：谭建新[1] 刘郴州[1] 王优[1] 黄宇戈[1] 黄秀兰[1] 方希敏[1] 

机构地区：[1]广东医学院附属医院儿科,广东湛江524001

出　　处：《中国病理生理杂志》2004年第9期1631-1633,共3页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目(No.020566)

摘　　要：目的探讨钙调神经磷酸酶(CaN)在大鼠慢性缺氧性右心室肥大中的作用以及L-型钙通道阻滞剂对其的影响。方法将大鼠置于氧浓度为(10±05)%的大型玻璃舱中每天24h缺氧持续共7d建立大鼠慢性缺氧模型。实验大鼠分为3组,每组各20只,即正常组、缺氧组、苯磺酸氨氯地平(norvasc)处理组,缺氧组持续缺氧21d,norvasc处理组在缺氧的同时灌喂norvasc30mg·k-1·d-1。第21d每组取10只大鼠分离心脏称重,并测CaN活性水平,另10只取心脏测定心肌细胞[Ca2+]i。结果(1)缺氧组右室与左室加室间隔的重量比、右室重/体重均明显高于正常组和norvasc处理组(均P<001)。(2)缺氧组右心室心肌细胞[Ca2+]i明显高于正常组(P<001),而norvasc处理组则明显低于缺氧组(P<001)。(3)缺氧组右心室心肌CaN活性明显高于正常组(P<001),而norvasc处理组则明显低于缺氧组(P<001)。结论CaN可能参与了大鼠慢性缺氧性右心室肥大,L-型钙通道阻滞剂可能是通过降低心肌细胞内钙浓度,调控CaN活性而阻滞慢性缺氧右心室肥大。AIM: To study the role of calcineurin in the progression of right ventricle cardiac hypertrophy in the chronic hypoxia rats and examine the effect of Ca 2+ channel blockers on the activation of calcineurin. METHODS: Sixty rats were divided into three groups: treatment group with amlodipine besylate ablets, chronic hypoxia group, normal control group with normal oxygen. The rats in treatment group and chronic hypoxia group were exposed to normobaric chronic hypoxia(10±0 5)% O 2 for 21 days. All hearts were removed immediately after dissection for further investigation. RESULTS: (1)The RV/(LV+S),RV/BW were significantly higher in hypoxia group than that of control group and treatment group( P <0 01,respectively); (2) Right ventricular cardiomyocytes [Ca 2+ ] i in treatment group were significantly higher than that of control group and lower that that of hypoxia group( P <0 01,respectively); (3) The activity of calcineurin of the heart in hypoxia group were significantly increased when compared with control group. Amlodipine besylate ablets apparently suppressed the activity of calcineurin( P <0 01). CONCLUSION: Calcineurin possibly plays a role in the progression of right ventricle cardiac hypertrophy in the chronic hypoxia rats;Blockade of L-type Ca 2+ channels with amlodipine besylate ablets effectively prevents its development possibly by inhibition of calcium inflow and suppression of the calcineurin activity. [

关 键 词：钙神经素 缺氧 心脏扩大 氨氯地平 

分 类 号：R363[医药卫生—病理学]