雌激素对大鼠血管平滑肌细胞囊泡素-1基因表达的影响  

Effect of estrogen on the gene expression of caveolin-1 in rat vascular smooth muscle cells

作　　者：杨发林[1] 刘贤锡[1] 贺红[2] 张建业[1] 许伟华[3] 张岩[1] 

机构地区：[1]山东大学医学院生物化学和分子生物学研究所,齐鲁医院检验科山东济南250012 [2]齐鲁医院心内科,山东济南250012 [3]齐鲁医院第二医院内科,山东济南250012

出　　处：《中国病理生理杂志》2004年第9期1638-1641,共4页Chinese Journal of Pathophysiology

摘　　要：目的探讨雌激素对血管平滑肌细胞囊泡素-1基因表达的影响。方法取Wistar雌性大鼠,分为3组假手术组,卵巢切除后皮下埋植雌激素组(OVX+E组)及卵巢切除后皮下埋植安慰剂组(OVX+V组)。用药2周后处死大鼠,剥离主动脉平滑肌组织,提取总RNA进行半定量RT-PCR分析,检测雌激素对囊泡素-1(caveolin-1)基因表达的影响。为进一步明确雌激素是否直接调节血管平滑肌细胞caveolin-1基因表达,又采用100nmol/L17β-雌二醇(17β-E2)处理培养的大鼠血管平滑肌细胞24h,通过Northernblot分析检测雌激素对细胞caveolin-1mRNA表达的影响。结果OVX+E组大鼠主动脉平滑肌组织caveolin-1基因表达量明显高于OVX+V组,17β-E2处理的培养细胞中caveolin-1基因mRNA表达量高于未用药的培养细胞。结论雌激素可促进血管平滑肌细胞caveolin-1基因表达,反映了雌激素心血管作用机理的一个方面。AIM: To assess the effect of estrogen on the gene expression of caveolin-1 in rat vascular smooth muscle cells (VSMCs). METHODS: Wistar rats were ovariectomized and subjected to subcutaneous implantation of placebo pellets (OVX+V group) or estradiol pellets (OVX+E group). 2 weeks after implantation, the expression of caveolin-1 gene in endothelium-denuded aortic tissue was examined by RT-PCR. Furthermore, Northern blotting was used to analyze the mRNA expression of caveolin-1 in cultured rat VSMCs. RESULTS: RT-PCR showed that expression of caveolin-1 gene was significantly higher in OVX+E group than that in OVX+V group. Northern blot analysis showed that the mRNA expression of caveolin-1 was higher in VSMCs pretreated with 17β-estradiol (17β-E 2) than that in VSMCs without 17β-E 2 pretreatment. CONCLUSION: Estrogen up-regulates the gene expression of caveolin-1 in the vascular wall, partially indicating the cardiovascular effect of estrogen. [

关 键 词：雌激素 细胞质膜微囊蛋白 血管平滑肌细胞 

分 类 号：R363[医药卫生—病理学]