叶绿酸铜钠抗肿瘤作用的体外实验研究  

Antitumor effect of chlorophyllin in vitro

作　　者：丁小文[1] 郑树[1] 彭佳萍[1] 吕庆华[1] 黄建[1] 

机构地区：[1]浙江大学肿瘤研究所

出　　处：《中国病理生理杂志》2004年第9期1659-1663,共5页Chinese Journal of Pathophysiology

摘　　要：目的研究叶绿酸铜钠(CHL)体外抑制HT29肠癌细胞的作用及机理。方法①MTT法测半数抑制浓度(IC50)及生长曲线;②Wright-Giemsa染色、DNA电泳和流式细胞仪观察CHL对HT29细胞凋亡的诱导作用;③PCR-ELISA方法定量定性检测CHL对HT29细胞端粒酶的影响;④RT-PCR、Westernblot方法检测环氧化酶2(COX-2)的表达。结果①CHL能抑制HT29细胞生长且呈剂量依赖关系;②CHL能够使HT29细胞阻滞在G1期,但并不能诱导细胞凋亡;③CHL能够抑制HT29细胞端粒酶活性和COX-2的表达。结论CHL能抑制HT29细胞生长,这种抑制作用同端粒酶活性抑制、COX-2表达下调及细胞G1期阻滞有关,但与细胞凋亡无关。AIM: To study the effect and mechanism of chlorophyllin (CHL) inhibiting HT29 cells. METHODS: IC 50 value and growth curve of HT29 cells were detected with MTT method. Apoptosis was detected with Wright-Giemsa staining, FCM and DNA electrophoresis. Telomerase was detected by PCR-ELISA, and protein and mRNA expression of COX-2 gene were detected through RT-PCR and Western blot. RESULTS: CHL inhibited the growth of HT29 in a dose-dependent manner. CHL blocked HT29 cells in G 1 phase but did not induce apoptosis. Different concentration of CHL inhibits the expression of telomerase and COX-2 in HT29 cells. CONCLUSION: CHL inhibits the growth of HT29 cells by inhibiting the expression of telomerase and COX-2 and blocking cells in G 1 phase. [

关 键 词：叶绿素 端粒 末端转移酶 结直肠肿瘤 细胞凋亡 

分 类 号：R363[医药卫生—病理学]