酵母双杂交研究肾癌中Wnt/Frizzled信号通路的传导  

Wnt/Frizzled signaling pathway in renal carcinoma

作　　者：龚侃[1] 林桂亭[1] 张志文[2] 常智杰[3] 那彦群[1] 

机构地区：[1]北京大学泌尿外科研究所北京大学第一医院泌尿外科,100034 [2]北京大学医学部生理系 [3]清华大学生命科学院

出　　处：《中华医学杂志》2003年第14期1270-1273,共4页National Medical Journal of China

摘　　要：目的　为研究肾癌中Wnt/Frizzled信号通路的传导 ,尤其是TCF4 (TCellFactor)的作用。方法　构建肾癌酵母双杂交文库及hTCF4酵母双杂交表达载体 ;通过酵母双杂交技术 ,以hTCF4为诱饵蛋白 ,自肾癌酵母双杂交文库中钓出与hTCF4相互作用的蛋白。结果　共钓出与hTCF4相互作用的阳性克隆 6 7个 ,其中包括 β 连环蛋白 ,TCF4,转录激活因子 5 ,锌指蛋白和人类基因组文库克隆 1980等。结论　肾癌中存在Wnt/Frizzled信号通路 ,其重要信号分子hTCF4与 β 连环蛋白相互作用 ,尚可以自身相互作用形成同源二聚体或同源共聚体 ,从而发挥其转录因子的作用。Objective To investigate the transduction of Wnt/Frizzled signaling pathway, especially the function of T cell factor 4 (TCF 4), in renal carcinoma. Methods A renal carcinoma yeast two hybrid library and a human TCF 4 yeast two hybrid expression vector were constructed. Proteins interacting with the bait protein human TCF 4 were obtained from the renal carcinoma yeast two hybrid library by reverse yeast two hybrid system. Results 67 positive clones interacting with the bait protein TCF 4 were obtained by reverse yeast two hybrid system, including 18 beta-catenin clones, 24 TCF 4 clones and 25 unknown clones. Conclusion Wnt/Frizzled signaling pathway exists in renal carcinoma. TCF 4, its important signal factor, interacts with beta- catenin and forms homodimer or homocopolymer by itself, thus displaying its constitutive transcriptional activity.

关 键 词：酵母双杂交技术 肾癌 Wnt/Frizzled 信号传导 T细胞因子4 肿瘤治疗学 

分 类 号：R737.11[医药卫生—肿瘤]