SV40转化的人胃黏膜上皮细胞与胃癌细胞间的比较蛋白质组学研究  

Comparative proteome analysis between SV40 transferred cells of human gastric mucosa epithelial and human gastric cancer

作　　者：贾继辉[1] 陈春燕[2] 于修平[1] 郭辉玉[3] 张茂修[1] 周亚滨[1] 栾怡[1] 齐眉[1] 赵蔚明[1] 

机构地区：[1]山东大学医学院微生物学教研室 [2]山东大学第二医院 [3]中山大学中山医学院微生物学教研室

出　　处：《中华微生物学和免疫学杂志》2004年第3期226-229,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金资助项目 ( 3 0 2 70 0 79)

摘　　要：目的　研究猴病毒 4 0 (SV4 0 )转化的人胃黏膜上皮细胞GES 1与人胃癌细胞MGC 80 3的差异蛋白质组。方法　运用固相pH梯度双向凝胶电泳技术比较两者差异蛋白 ,差异蛋白用胰酶进行胶内酶切 ,肽混合物使用基质辅助激光解吸 /电离飞行时间质谱仪 (MALDI TOF MS)进行质谱分析 ,将肽质量指纹谱数据输入互联网上的蛋白质数据库进行检索。结果　获得 17个GES 1和MGC 80 3细胞间表达差异蛋白质的明确信息 ,涉及细胞骨架、细胞调控、细胞凋亡和生物氧化等种类蛋白质。结论　SV4 0转化的人胃黏膜上皮细胞GES 1与人胃癌细胞MGC 80 3间的表达蛋白具有差异 ,这些差异蛋白分析有助于深入研究胃癌发生发展机制 。Objective To study differential proteome between SV40 transformed GES 1 cells of human gastric mucosa epithelial and human gastric cancer MGC 803 cells. Methods Two dimensional gel electrophoresis(2 DE) maps of the proteins extracted from MGC 803 and GES 1 were applied respectively. The differential expression proteins were digested in gel by enzyme and identified with assisted laser desorption ionization time of flight mass spectrometry (MALDI TOF MS). The data obtained from peptide mass fingerprinting (PMF) were searched using the Internet available database. Results 17 alterated protein spots were successfully identified. These proteins were involved in cytoskeletons, regulation of gene expression,apoptosis,biological oxidation and other aspects. Conclusion These proteins from SV40 transferred GES 1 cells of human gastric mucosa epithelial and human gastric cancer MGC 803 cells were differentially expressed. It will be helpful to further research of carcinogenisis and find new molecular markers for early diagnosis and treatment of gastric cancer.

关 键 词：胃黏膜上皮细胞癌 比较蛋白质组学 猴病毒40 早期诊断 病原微生物 

分 类 号：R735.2[医药卫生—肿瘤]