藏族群体7个Y-STR基因座及单倍型的遗传多态性  

The genetic polymorphism of 7 Y-STR loci and haplotype in Tibetan Population

作　　者：赵东[1] 王保捷[1] 丁梅[1] 卢岩[1] 孙大成[1] 刘志芳[1] 

机构地区：[1]中国医科大学法医学院,辽宁沈阳110001

出　　处：《法医学杂志》2004年第2期88-91,共4页Journal of Forensic Medicine

基　　金：辽宁省科委科学基金资助项目(辽科发9810500601);辽宁省教育厅科学基金资助项目(教科发20054552);沈阳市科学技术局科学基金资助项目(沈科发2002-41)

摘　　要：目的调查藏族Y染色体7个STR基因座及单倍型的遗传多态性。方法应用PCR、变性聚丙烯酰胺凝胶电泳结合银染显色分型技术,检测67例藏族男性DNA样品。结果在DYS393、DYS389Ⅰ、DYS19、DYS390、DYS389Ⅱ、DYS392等6个基因座中共检出25个等位基因,DYS385基因座检出22个等位基因组,频率分布在0.0149～0.5075之间,各基因座DP值分布在0.5075～0.8991之间,以DYS385基因座最高。由7个基因座组成的YH6系统单倍型有51种,DP值为0.9887,DYS393与DYS389Ⅱ基因座等位基因少,推测藏族群体在进化过程中可能发生过瓶颈事件。结论上述7个STR基因座属于高鉴别能力基因座,单倍型具有很高的遗传多态性。Objective To investigate the genetic polymorphism of 7 Y-STR loci and haplotypes in Tibetan population. Methods The DNA samples from 67 Tibetan men were analyzed by PCR and the denatured PAGE followed by silver staining. Results 25 alleles were identified for 6 loci including DYS393 ,DYS389Ⅰ,DYS19, DYS390, DYS389Ⅱ and DYS392, 22 alleles were identified for DYS385, the frequency distributions were between 0.014 9～0.507 5, the DP value distributions were between 0.507 5～0.899 1, the DP value of DYS 385 was the highest. 51 haplotypes were identified for the YH6 system which consisted of the 7 loci, the DP value of this system was 0.988 7, the alleles for DYS393 and DYS389Ⅱ were few ,it followed that perhaps Tibetan population had met with bottleneck event in their evolution process. Conclusion The 7 STR loci above belong to loci of high discriminating ability, the haplotypes are highly polymorphic.

关 键 词：藏族 Y-STR基因座 单倍型 遗传多态性 法医学 染色体 

分 类 号：R394[医药卫生—医学遗传学] D919[医药卫生—基础医学]