氦氖激光对新生大鼠缺血缺氧性脑损伤的影响  

Effect of He-Ne laser on brain injury after hypoxia-ischemia in newborn rats

作　　者：刘伟[1] 吴爱群[2] 李宛青[3] 高艳[4] 张华[4] 汤善均[4] 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所解剖与组织胚胎学系,北京市100005 [2]解放军第二军医大学解剖教研室,上海市200433 [3]郑州师范高等专科学校生命科学系,河南省郑州市450015 [4]郑州大学基础医学院人体解剖教研室,河南省郑州市450052

出　　处：《中国临床康复》2004年第16期3058-3059,共2页Chinese Journal of Clinical Rehabilitation

基　　金：河南省科技攻关项目(0211043800)~~

摘　　要：目的:探讨氦氖激光穴位照射对新生鼠缺血缺氧性脑损伤的作用及其可能机制。方法:42只7d龄Wistar大鼠,单纯随机分为3组:假手术对照组、缺血缺氧组、氦氖激光穴位照射组,常规饲养22d后,取左侧脑组织进行常规石蜡包埋、切片、0-乙酰转移酶(cholineacetyltransferase,ChAT)和脑源性神经营养因子(brain-derivedneurotrophicfactor,BDNF)免疫组织化学染色。结果:氦氖激光穴位照射可明显改善缺血缺氧后脑神经元内尼氏体的脱失现象,增加缺血缺氧后脑神经元内胆碱ChAT和BDNF的表达。结论:氦氖激光穴位照射对缺血缺氧后脑组织具有神经保护效应,其作用机制推测与促进神经元对BDNF的表达有关。AIM:To study the role and possible mechanism of He-Ne laser acupoint irradiat ion treatment on hypoxia ischemia brain damage(HIBD). METHODS:Forty-two Wistar rats,7 days of age,were randomized into sham-operat ion group,HIBD group and He-Ne laser acupoint irradiation group(laser group).Tw enty-two days later,left brains were taken out and made into paraffin sections. Immunohistochemical staining was performed with choline acetyltransferase(ChAT) and brain-derived neurotrophic factor(BDNF). RESULTS:He-Ne laser acupoint irradiation was effective in inhibiting the loss of Nissl bodies in brain tissue and promoting the expression of ChAT and BDNF i n brain tissue after hypoxia-ischemia. CONCLUSION:He-Ne laser acupoint irradiation has a neuroprotective effect on b rain after hypoxia-ischemia,and its action mechanism may be related to the expr ession of BDNF.

关 键 词：脑损伤 激光 大鼠 低氧-缺血 脑源性神经营养因子 胆碱0-乙酰转移酶 

分 类 号：R743[医药卫生—神经病学与精神病学]