胃转安方对胃癌前期病变大鼠端粒酶活性干预作用的实验研究  

Experimental study of Weizhuan’an prescription Interference Effect with Telomerase activity of Rats Gastric Precancerous Lesions

作　　者：刘华一[1] 王蓉[1] 王秀娟[1] 江海涛[2] 

机构地区：[1]天津市中医药研究院附属医院,天津300020 [2]天津中医学院,天津300193

出　　处：《中医药学刊》2004年第7期1253-1254,共2页Study Journal of Traditional Chinese Medicine

基　　金：天津市自然科学基金项目 ( 0 0 3 610 611)

摘　　要：目的 :探讨胃癌前期病变端粒酶活性以及中药胃转安方对其干预作用 ,探索对胃癌前期病变以端粒酶为靶基因的中医药治疗方法。方法 :Wistar大鼠 64只 ,随机分为正常组、模型组、中药组、自然恢复组。正常组和自然恢复组各 11只 ,模型组和中药组各 2 1只。除正常组外 ,各组均采用MNNG饮水 +雷尼替丁法造模 ,2 6周后停止造模。中药组用掺入胃转安方的饲料饲养 ,至 3 8周处死各组大鼠取材检测。结果 :正常组 1例、模型组 4例、自然恢复组 2例端粒酶阳性 ;中药组无 1例阳性。结论 :经MNNG造模 ,在出现胃癌前期病变的同时 ,端粒酶可被激活。中药可有效抑制端粒酶活性 ,从而阻断胃癌前期病变的发展 ,并有可能通过抑制端粒酶活性对胃癌进行干预治疗。Objective: To investigate telomerase activity of gastric precancerous lesions and Interference effect of Traditional Chinese medicinal Weizhuan'an prescription . Methods: 64 Wistar rats were randomly divided into the normal group ,model group, TCM group, and spontaneous recovery group . The normal group and the spontaneous recovery group are 11 rats for each , the model group and the TCM group are 21 rats for each. Excepting normal group, all groups were made into modeling though MNNG for 26 weeks. TCM group treaded with Weizhuan'an prescription . To 38 weeks, all rats were killed and take stomach tissues to examine. Result: Normal group one rat, model group four rat and spontaneous recovery group two rats were measured to positive telomerase. TCM group wasn't measured to positive telomerase. Conclusion: when gastric precancerous lesions emerged, the telomerase can be activated. TCM can inhibited telomerase activity and blocked gastric precancerous lesions. Perhaps we can treat stomach cancer by inhibiting telomerase activity.

关 键 词：胃癌前病变 端粒酶 胃转安方 

分 类 号：R730[医药卫生—肿瘤]