类金刚石薄膜蛋白吸附与碳相成分关系的定量分析研究  

Analysis of Correlation Degree Between Phase Components and Adsorptive Amounts of Serum Proteins for DLC Film

作　　者：李伯刚[1] 康云清[1] 尹光福[1] 郑昌琼[1] 

机构地区：[1]四川大学材料科学与工程学院,成都610065

出　　处：《生物医学工程学杂志》2004年第4期549-553,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目 ( 3 9870 2 3 2 );教育部博士点基金资助项目 ( 10 0 0 610 0 4)

摘　　要：运用灰色系统理论中的 T型关联度分析方法 ,对类金刚石 (DL C)薄膜、富石墨相 DL C薄膜和富金刚石相 DL C薄膜三种 DL C薄膜进行了碳相成分对其白蛋白 (HSA)、纤维蛋白原 (HFG)、免疫球蛋白 (Ig G)三种血浆蛋白吸附量影响的定量分析研究。合理地解释了三种材料蛋白吸附量随碳相成分变化的实验结果 ,并得出如下重要的分析结论 :(1)石墨和 C- H相对 HSA的吸附影响较大 ,随着二者的增加 ,HSA的吸附量下降 ;(2 )与 HFG吸附有较强关联的碳相成分是 DL C相和 C- O相 ,前者呈负相关 ,后者为正相关 ;(3)各碳相成分对 Ig G的吸附均有性质不尽相同的影响 ,但程度有限 ,且彼此间相差不大 ;(4 ) DL C碳相具有增强 HSA吸附、抑制 HFG、Ig G吸附的双重功效 ,其对 DL C薄膜血液相容性的影响远较其它碳相成分更为重要。In the present paper,the influence of carbon phase components of three kinds of diamond like carbon (DLC) films,viz. DLC,DLC rich in graphite and DLC rich in diamond films,on adsorption of human serum albumin (HSA),human serum fibrinogen (HFG) and immunoglobin (IgG) was quantitatively analyzed by use of T-type correlation degree in the grey system theory. Through the analysis,the rational explanation for adsorptive amounts variations of the serum proteins with phase components in the experiment is reached and some essential conclusions have been obtained: (1) The effect of graphite phase and C-H phase on HSA adsorption are greater than that of other phase components;with the increase of these two phase coumponents,the adsorptive amounts of HSA decrease;(2) The powerful influence on HFG adsorption stems from DLC phase and C-O phase;with the decrease of DLC phase or the increase of C-O phase,the adsorptive amounts of HFG increase;(3) All of the carbon phase components have only limited influence on IgG adsorption in positive or negative fashion with a little difference in degree;(4) DLC phase has both effects of enhancing adsorption for HSA and weakening adsorption for HFG and IgG, thus its influence on the hemocompatibility of DLC films is much more important than that of other phase components.

关 键 词：类金刚石薄膜 蛋白吸附 碳相成分 血液相容性 白蛋白 纤维蛋白原 免疫球蛋白 

分 类 号：R318.08[医药卫生—生物医学工程]