血管生成抑制剂SU5416对大鼠胰腺癌生长和转移的抑制作用  

Inhibition of angiogenesis inhibitor SU5416 on growth and metastasis of experimental rat pancreatic cancer

作　　者：侍立志[1] 王兆春 陈紫平[1] 邹桂华[3] 所剑[4] 

机构地区：[1]深圳市宝安人民医院普外科,518101 [2]深圳市松岗人民医院外科 [3]深圳市宝安人民医院病理科,518101 [4]吉林大学第一医院普外科

出　　处：《中华普通外科杂志》2004年第7期404-406,共3页Chinese Journal of General Surgery

摘　　要：目的研究血管生成抑制剂SU5 4 16对大鼠胰腺癌生长和转移的抑制作用。方法采用二甲基苯丙葱直接置入大鼠胰腺 ,建立临床类似胰腺癌的大鼠模型。将 6 0只胰腺癌SD大鼠随机分为 4组 ,分别自腹腔注射生理盐水 (对照组 )、5氟脲嘧啶 (5 Fu组 )、SU5 4 16制剂 (SU5 4 16组 )和5 Fu +SU5 4 16联合用药 (联合组 ) ,隔日 1次。连续 13周后处死大鼠 ,剖腹测量原位肿瘤瘤重、抑瘤率、肿瘤微血管密度、胰腺癌细胞凋亡指数 ,同时观察腹膜、肝脏及毗邻脏器转移和腹水情况。结果对照组、5 Fu组、SU5 4 16组和 5 Fu +SU5 4 16联合组诱发肿瘤瘤重分别为 (1 15± 0 2 1)g、(0 6 8±0 4 2 ) g、(0 31± 0 11)g、(0 19± 0 0 6 ) g ;抑瘤率分别为 0、4 8%、80 %、85 % ;肿瘤微血管密度分别为12 3± 3 2、11 4± 3 8、2 1± 1 5、1 8± 1 1;凋亡指数分别为 (2 6± 1 9) %、(5 7± 3 1) %、(13 2±4 3) %、(2 1 1± 7 2 ) % ;腹膜转移率分别为 83%、4 6 %、2 5 %、0 ;肝转移率分别为 6 7%、2 7%、17%、0。实验组与对照组比较差异具有显著意义 (P <0 0 5 )。结论SU5 4 16因能抑制依赖于血管表皮生长因子刺激的血管表皮细胞增殖而抑制胰腺癌的生长和转移 ,化疗药物联合应用具有协同作用。Objective To study the effects of angiogenesis inhibitor SU5416 on the growth and metastasis of pancreatic cancer in SD rat model. Methods Dimethylbenzanthracine (9 mg) (DMBA),was implanted into the parenchyma of Sprague Dawley rat pancreas to induce pancreatic cancer. Rats with established pancreatic carcinoma were randomly divided into 4 groups (15 rats each) to receive every the other day for consecutive 13 weeks before sacrifice peritoneal cavity injection of: Normal saline (control),5-fluorouracil 30 mg·kg -1 (5-Fu group),SU5416 16 mg·kg -1 (SU5416 group),and both 5-Fu and SU5416(combined treatment group). Tumor weight,inhibition rates,intratumoral microvessel density (MVD),apoptotic index (AI) and metastasis were evaluated. Results Tumor weight was (1.15±0.21) g,(0.68±0.42) g,(0.31±0.11) g,(0.19±0.06) g respectively;the inhibition rate was 0,48%,80%,85% respectively;the intratumoral microvessel density (MVD) was (12.3±3.2),(11.4±3.8),(2.1±1.5),(1.8±1.1) respectively;The apoptotic index (AI) was (2.64±1.86)%,(5.71±3.14)%,(13.21±4.26)%,(21.12±7.15)% respectively. Peritoneal metastasis was significantly less severe in 5-Fu group,SU5416 group and combined group than that in control group(83% versus 46%,25% and 0) ( P <0.05). Liver metastasis rate significantly decreased in the three treatment groups (67% vs 27%,17% and 0,P <0.05). ConclusionsSU5416 inhibits the multiplication of vascular endothelial growth factor (VEGF) hence impedes the growth and metastasis of rat pancreas cancer.

关 键 词：血管生成抑制剂 SU5416 大鼠 胰腺癌 肿瘤生长 肿瘤转移 抑制作用 

分 类 号：R735.9[医药卫生—肿瘤]