伏格特-小柳-原田综合征患者外周血淋巴细胞Fas和FasL mRNA的表达  

Increased expression of Fas and FasL mRNA in peripheral blood lymphocytes in Vogt-Koyanagi-Harada syndrome

作　　者：陈玲[1] 杨培增[1] 周红颜[1] 黄祥坤[1] 钟晖[1] 谢楚芳[1] 

机构地区：[1]中山大学中山眼科中心

出　　处：《中华眼科杂志》2004年第8期507-509,共3页Chinese Journal of Ophthalmology

基　　金：国家杰出青年基金资助项目(39925034);广东省自然科学基金资助项目(980114)

摘　　要：目的 探讨Fas、FasL mRNA在伏格特-烛柳-原田(Vogt-Koyanagi-Harada,VKH)综合征患者外周血淋巴细胞的表达。方法 于2000年1～6月抽取16例VKH综合征患者和19例正常人的外周血,提取淋巴细胞的总RNA,将RNA逆转录为cDNA,然后用荧光定量聚合酶链反应(fluorescent quantitative polymerase chain reaction,FQ-PCR)的方法实时检测Fas、FasL PCR扩增的全过程,计算样本中Fas、FasL mRNA的表达量。结果 VKH综合征患者外周血淋巴细胞的Fas mRNA的表达量[(1.6±2.0)×106]显著高于正常对照组[(5.7±2.0)×105](t=4.50,P<0.05),FasLmRNA的表达量[(1.8±1.5)×106]也显著高于正常对照组[(4.8±3.5)×105](t=9.57,P<0.05)。结论 VKH综合征患者外周血中长期存在的大量Fas、FasL mRNA高表达的活化自身免疫性淋巴细胞,可能是其炎性反应反复发作、病情迁延不愈的重要原因之一。(中华眼科杂志,2004,40:507-509)Objective To evaluate the expression of Fas and FasL messenger RNA ( mRNA) in peripheral blood lymphocytes and its possible role in the pathogenesis of Vogt-Koyanagi-Harada syndrome. Methods Blood samples were obtained from 16 patients with Vogt-Koyanagi-Harada syndrome and 19 healthy individuals from January to June, 2000. Total RNA was extracted from peripheral blood lymphocytes. cDNA was prepared from RNA with oligo d(T)18 and M-MuLV reverse transcriptase. The expression of Fas mRNA and FasL mRNA in peripheral blood lymphocytes was determined using fluorescent quantitative polymerase chain reaction ( FQ-PCR) . Amplification was carried out through 32 cycles, 30 seconds denaturation at 931 , 30 seconds annealing at 58℃ , and 45 seconds primer extension at 721. The final extension time was 7 minutes. Results The expression of Fas mRNA on peripheral blood lymphocytes [( 1.6 ±2.0) ×106] was significantly higher than that of the controls [ (5. 7 ±2. 0) × 105 ] (t=4. 50,P< 0. 05). The mRNA expression of FasL on peripheral blood lymphocytes [(1.8±1.5) × 106 ] was also higher than that of the controls [ (4. 8 ± 3. 5 ) × 105 ] (t = 9. 57, P < 0. 05 ). Conclusions A high level of Fas mRNA and FasL mRNA expression in activated peripheral blood lymphocytes is observed in patients with Vogt-Koyanagi-Harada syndrome. These long-persisted activated lymphocytes may be responsible for the perpetuation and recurrence of Vogt-Koyanagi-Harada syndrome. ( Chin J Ophthalmol, 2004,40:507-509)

关 键 词：伏格特-小柳-原田综合征 外周血 淋巴细胞 FAS FASL MRNA表达 葡萄膜炎 

分 类 号：R773[医药卫生—眼科]