视网膜色素上皮受体介导的内吞作用  

Receptor mediated endocytosis of retinal pigment epithelial cell

作　　者：李若梅[1] Sorensen K.KAREN Smedsrod BARD 刘熙朴[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院眼科眼科研究中心,北京100730 [2]Department of Experimental Pathology Institute of Medical Biology University of Tromso Norway

出　　处：《中华眼科杂志》2004年第8期539-544,共6页Chinese Journal of Ophthalmology

摘　　要：目的 研究视网膜色素上皮(RPE)细胞内吞光感受器细胞外基质(IPM)内大分子可溶性物质的可能性及其机制。方法 采用荧光标记的甲醛作用后的血清白蛋白(F-FSA),作为清道夫受体配体的指示物,与组织块培养的猪RPE细胞共同孵育,用荧光显微镜和电子显微镜观察RPE细胞对F-FSA的内吞情况。结果 RPE细胞能够摄入F-FSA;这一作用随配体浓度增加而提高,随孵育时间延长而增强;相同或相似的配体之间存在竞争作用;F-FSA进入RPE细胞后,以微囊泡的形式存在丁细胞中。结论 RPE细胞能够摄人携带负电荷的大分子物质,这一作用是通过受体介导的内吞作用完成的,清道夫受体可能是介导这一作用的受体;受体介导的RPE细胞的内吞作用可能是IPM内大分子物质代谢的重要机制。(中华眼科杂志,2004,40:539-544)Objective To evaluate the possibility and mechanism of the turnover of the negatively charged macromolecule by retinal pigment epithelial ( RPE) cells. Methods Cultured porcine RPE eye cups were incubated with fluorescence labeled formaldehyde treated serum albumin ( F-FSA) , a classical ligand for scavenger receptors. The endocytosis of F-FSA by RPE cells under different conditions was evaluated systematically by fluoresecent microscopy and electron microscopy. Results The amount of F-FSA ingested by RPE cells depends on the incubation time and the concentration of ligand. Similar ligands compete for the binding site of RPE cells. Small vesicles containing F-FSA are noticed in the cytosol of RPE cells as demonstrated by electron microscopy. Conclusion The RPE cells can effectively ingest negatively charged macromolecules, possibly by scavenger receptor mediated endocytosis. (Chin J Ophthalmol, 2004, 40-.539-544)

关 键 词：视网膜色素上皮受体 内吞作用 光感受器 细胞外基质 大分子可溶性物质 

分 类 号：R77[医药卫生—眼科]