四种细胞因子对牛晶状体上皮细胞胶原合成的影响及对前胶原mRNA表达的调控  

Effect of cytokine and sandostatin on collagen synthesis and expression of procollagen mRNA by lens epithelial cells

作　　者：张凤妍[1] 王国军[2] 

机构地区：[1]郑州大学附属第一医院眼科,450052 [2]华中科技大学同济医学院

出　　处：《中华眼科杂志》2004年第8期545-548,共4页Chinese Journal of Ophthalmology

摘　　要：目的 探讨表皮生长因子(EGF)、白细胞介素1(IL-1)、γ干扰素(IFN-γ)和生长抑素8肽(SS-8肽)在晶状体后囊膜混浊形成中的作用及影响胶原合成的机制。方法 细胞接种于96孔培养板贴壁后分别加入含有浓度为10～2-102ng/ml的EGF、10-103ng/ml的IL-1、10-11-10-7mol/L的SS-8肽及10-105U/ml的IFN-γ细胞培养液,采用3H-脯氨酸掺入法检测对胶原合成的影响;用Northern blot技术检测对Ⅰ、Ⅲ型前胶原mRNA表达的调控。结果 EGF 10～1-102ng/ml、IL-1 102～105ng/ml显著促进胶原合成,IFN-γ 103～105U/ml、SS-8肽10-10～10-7mol/L显著抑制胶原合成;1 ng/ml的EGF及103ng/ml的IL-1可增加Ⅰ、Ⅲ型前胶原mRNA表达,而103U/ml的IFN-γ及10-9mol/L的SS-8肽可减少Ⅰ、Ⅲ型前胶原mRNA表达。结论EGF、IL-1参与了晶状体后囊膜混浊形成中的胶原合成,且对胶原合成的影响是作用于转录水平;IFN-γ、SS-8肽的作用提示其可用于防治晶状体后囊膜混浊。 (中华眼科杂志,2004,40:545-548)Objective To study the effects of epidermal growth factor (EGF), interleukin-1 (IL-1) , interferon-γ ( IFN-γ) and sandostatin on collagen synthesis and expression of types Ⅰ and Ⅲ procollagen mRNA by bovine lens epithelial cells in vitro. Methods Cells were plated in 96-well tissue culture plates. After attached, serum-free medium containing EGF 10-2-102 ng/ml, IL-1 10-105 ng/ml, sandostatin 10-11-10-7 mol/L and IFN-γ 10-105 U/ml were added into the plates. Collagen synthesis was measured with 3H-proline incorperation, and expression of procollagen mRNA was detected with Northern blot. Results EGF 10-1-102 ng/ml and IL-1 102-105 ng/ml obviously promoted collagen synthesis. EGF 1 ng/ml and IL-1 10 ng/ml increased the expression of types Ⅰ and Ⅲ procollagen mRNA. IFN-γ 103-105 ng/ml and sandostatin 10-10-10-7 mol/L decreased collagen synthesis. IFN-γ 103 U/ml and sandostatin 10 -9 mol/L decreased the expression of types Ⅰ and Ⅲ procollagen mRNA. Conclusions EGF and IL-1 may enhance the occurrence of posterior capsular opacification, they affect types Ⅰ and Ⅲ procollagen gene expression at transcription level. IFN-γ and sandostatin might be used to prevent the occurrence of posterior capsular opacification. (Chin J Ophthalmol, 2004, 40:545-548)

关 键 词：细胞因子 牛 晶状体上皮细胞 胶原合成 前胶原 mRNA表达 表皮生长因子 白细胞介素1 Γ干扰素 生长抑素8肽 

分 类 号：R776[医药卫生—眼科]