鼠视网膜色素上皮显微移植方法的比较研究  

Comparison of microinjection methods for subretinal RPE transplantation in the rat

作　　者：周朝晖[1] Henry J.KAPLAN 

机构地区：[1]解放军总医院三○四临床部眼科,北京100037 [2]美国华盛顿大学视觉科学和眼科

出　　处：《中华眼科杂志》2004年第8期549-551,共3页Chinese Journal of Ophthalmology

摘　　要：目的 比较不同的视网膜色素上皮(RPE)移植进入视网膜下腔的显微注射技术引起Lewis鼠宿主眼的医源性损伤。方法 Lewis鼠45只眼分为3组,视网膜下腔注射转染荧光素蛋白的hTERT-RPE1细胞。第一组使用Hamilton注射器。第二组使用Eppendorf微量加样器。第三组使用输入泵注射器。光学显微镜下评价宿主眼的医源性损伤程度。结果 使用注入泵注射器组12只眼无损伤,而使用Eppendorf微量加样器组和Hamilton注射器组均有14只眼发生了严重或者轻度损伤。结论 注入泵注射器的低速电机泵可提供缓慢且可以控制的液体注入流,能够明显减少RPE移植入视网膜下腔时对宿主眼的医源性损伤。(中华眼科杂志,2004,40:549-551)Objective To compare iatrogenic damage to the host eye caused by different injection techniques for transplantation RPE into the subretinal space of Lewis rats. Methods A total of 45 eyes from Lewis rats were divided into three groups and immortalized hTERT-RPE1 cells ( clontechniques) transfected with green fluorescent protein ( GFP) was injected into the subretinal space. The injection was given by using Hamilton syringe, Eppendorf repeat micro-pipettor, or a pediatric syringe infusion pump in different groups respectively. Iatrogenic damage to the host eye was evaluated by light microscopy. Results No damage to the host eye was observed in 12 of 15 eyes injected with the syringe infusion pump. In contrast, severe or mild damage was detected in 14 of 15 eyes after injections using either the Hamilton syringe or the Eppendorf micro-pipettor. Conclusions The syringe infusion pump minimizes iatrogenic damage to the host eye during RPE transplantation into the subretinal space of the rat. The slow and controlled fluid infusion provided by the low-geared electric motor of the pump is presumably beneficial during the surgical procedure. ( Chin J Ophthalmol, 2004, 40; 549-551 )

关 键 词：鼠 视网膜色素上皮移植 视网膜色素变性 显微镜检查 

分 类 号：R779.6[医药卫生—眼科]