阿司匹林治疗前后Alzheimer病患者外周血细胞因子基因表达变化  

Cytokins gene expression in the peripheral blood of patients with alzheimer disease before and after treatment of aspirin

作　　者：朱正禹[1] 毕建忠[1] 许顺良[1] 王晓云[1] 谢兆宏[1] 杨萧荟[2] 

机构地区：[1]山东大学第二医院,山东济南250033 [2]济南市第一人民医院

出　　处：《山东医药》2004年第22期3-5,共3页Shandong Medical Journal

摘　　要：目的　检测 Alzheim er病 (AD)患者使用非甾体类抗炎药阿司匹林前后外周血 Th1/Th2亚群五种细胞因子的基因表达变化 ,探讨其在 AD慢性病理过程中的作用及阿司匹林治疗 AD的机制。方法　应用 RT-RCR反应体系分别检测 AD患者使用阿司匹林前后及对照组外周血中白细胞介素 - 2 (IL - 2 )、白细胞介素 - 4 (IL -4 )、白细胞介素 - 6 (IL - 6 )、白细胞介素 - 10 (IL - 10 )、干扰素 -γ(IFN-γ)五种细胞因子的基因表达变化。结果　阿司匹林治疗前 AD患者 IL - 2、IL - 6表达明显上调 ,与对照组比较差异显著 (P<0 .0 5 ) ;治疗后 IL - 2、IL - 6的表达明显下调 ,与治疗前比较差异显著 (P<0 .0 5 ) ;治疗前后 IL- 4、IL- 10、IFN- γ的表达与对照组比较无显著差异 (P>0 .0 5 )。结论　 Th1/Th2亚群分泌的五种细胞因子可以作为检测 AD的外周血免疫标记物 ;Objective To measure five cytokines gene expression in the peripheral blood of AD patients,investigate their functions in the chronic pathologlic process in AD patients,and study the mechanism of nonsteroidal anti-inflammatory drugs(NSAID) on AD. Methods Using reverse transcription and polymerase chain reaction(RT-PCR) technique,we measured IL-2, IL-4, IL-6, IL-10 and IFN- γ gene expression in patients with AD (n=20) before and after treatment of aspirin and control subjects.Results Higher IL-2 and IL-6 gene expression was observed in the peripheral blood of AD compared with control subjects (P<0.05). Lower IL-2 and IL-6 gene expression after treatment of aspirin than before (P<0.05).IL-4, IL-10 and IFN- γ gene expression showed no difference among all subjects (P>0.05).Conclusion The five cytokines of Thl/Th2 lymphocyte subpopulations can be seemed as neuroimmno markers of AD patients in the peripheral blood.

关 键 词：阿司匹林 治疗 ALZHEIMER病 外周血 细胞因子基因 表达 

分 类 号：R749.16[医药卫生—神经病学与精神病学]