PDCD5蛋白在正常宫颈、宫颈上皮内瘤样病变和宫颈癌中的表达  

Expression of PDCD5 in tissues of normal cervix, CIN Ⅰ-Ⅲ and cervical cancer

作　　者：刘朝晖[1] 张岱[1] 李克敏[1] 廖秦平[1] 

机构地区：[1]北京大学第一医院妇产科,北京100034

出　　处：《北京大学学报（医学版）》2004年第4期407-410,共4页Journal of Peking University:Health Sciences

摘　　要：目的 :了解凋亡基因PDCD5在正常宫颈、宫颈上皮内瘤样病变 (cervicalintraepithelialneoplasia,CIN)和宫颈鳞癌中的表达 ,分析PDCD5在宫颈癌的发病中所起的作用。方法 :采用免疫组织化学的方法 ,对 93例宫颈组织 (其中包括正常宫颈 1 8例 ,CINⅠ级 1 9例、CINⅡ级 1 8例、CINⅢ级 2 0例、宫颈癌 1 8例 )的组织切片进行PDCD5的免疫组化染色。采用人工阅片评分及计算机辅助染色强度判定 2种方法判断宫颈组织PDCD5的染色阳性率及染色强度。采用单因素方差分析法判断PDCD5的表达与正常宫颈、CIN、宫颈鳞癌的相关性。结果 :免疫组织化学的结果显示 ,在正常宫颈及CINⅠ级组织中 ,PDCD5表达多呈强阳性 ,CINⅡ级以上及宫颈癌组织表达呈递减趋势。正常宫颈组织、CIN各级及宫颈癌组织之间PDCD5的平均光密度值分别为 0 .32 2、0 .36 6、0 .2 87、0 .2 5 2和 0 .2 0 6 ,各级病变之间差异均有显著性。在整体上说 ,PDCD5的表达在CINⅠ级较正常升高 ,在CINⅡ级以上随宫颈病变的进展呈下降趋势。结论Objective: To investigate the expression of PDCD5 in tissues of normal cervix, CIN Ⅰ-Ⅲ,cervical cancer and explore the relationship between PDCD5 and cervical cancer.Methods: After we defined the most fitful condition, tissues from 18 cases of normal cervix, 19 of CIN Ⅰ, 18 of CIN Ⅱ, 20 of CIN Ⅲ and 18 of cervical cancer were defined by indirect immunohistochemical technique. Positive expression rates and intensity of PDCD5 protein were investigated by observing under microscope and analyzing with computer imaging technique. The results were analyzed with one way anova. Results: The results of immunohistochemical staining showed that the percentage of strong positive cells in normal cervical tissue and CIN Ⅰ were significant higher than those of CIN Ⅱ, CIN Ⅲ and cervical cancer. On the whole of the condition of immunohistochemical staining, the expressions of PDCD5 were downregulated along the progression of cervical atypical epithelia, but that in CIN Ⅰ was upregulated.The ODs of normal cervix,CIN Ⅰ-Ⅲ,cervical cancer were 0.322,0.366,0.287,0.252,and 0.206 respectively.The intensity of each group showed obvious differences.Conclusion: We found that the expression of PDCD5 was upregulated in CIN Ⅰ and downregulated in CIN Ⅱ, CIN Ⅲ and cervical cancer. It suggests that PDCD5 is an important apoptosis regulating factor in the occurrence of cervical cancer.

关 键 词：PDCD5蛋白 宫颈上皮 瘤样病变 宫颈癌 基因表达 肿瘤 CIN 

分 类 号：R737.33[医药卫生—肿瘤]