大鼠软组织血管放射损伤动物模型的建立  

Establishment of an animal model of mice with radiation-injured soft tissue blood vessels

作　　者：王代友[1] 于大海[1] 温玉明[2] 巫家晓[1] 韦山良[1] 

机构地区：[1]广西医科大学口腔医学院,南宁530021 [2]四川大学华西口腔医学院

出　　处：《中华放射医学与防护杂志》2004年第4期302-303,共2页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目 (3 0 0 60 0 83 )

摘　　要：目的　建立大鼠软组织血管放射损伤模型。方法　选成年雄性Wistar大鼠 4 0只 ,以大鼠的右后肢为照射侧 ,左后肢作为对照侧 ,每只大鼠的总吸收剂量为 30Gy。放射治疗完成后第 1天行血管造影检查模型大鼠两侧后肢血管数目变化 ;取两侧的后肢肌肉组织标本行血管密度、平均截面积、平均管径测定及透射电镜血管超微结构检查。结果　血管造影图像分析显示右侧后肢血管数目少于左侧 ;血管染色病理图像分析表明右侧后肢肌肉组织血管密度、平均截面积、平均管径均低于左侧 ;透射电镜观察显示右侧后肢血管形态及内皮细胞超微结构同左侧相比均有明显的病理改变。结论　成功地建立了大鼠软组织血管放疗损伤模型。Objective The aim of this study was to establish an animal model of mice with radiation -injured soft tissue blood vessels. Methods Forty male mice were irradiated with 30 Gy on the right leg. After the irradiation was finished each of the 40 male mice was tested with angiography,and its muscle tissues on the bilateral legs were examined with vessel staining assay and electron microscopy. Results The results showed that the number of vessels on the right leg was less than that on the left leg ,the microvessel density,average diameter and average sectional aera of the right leg were all lower than those of the left ,and the configuration and ultra-structure of vessels were also different between both sides of legs. Conclusion In the study we successfully established an animal model of mice with radiation-injured soft tissue blood vessels.

关 键 词：大鼠 软组织 血管放射损伤 动物模型 恶性肿瘤 

分 类 号：R-332[医药卫生]