三磷酸甲酯中毒致母鸡脊髓神经元凋亡的研究  

Spinal cord neuronal apoptosis induced by triorthocresyl phosphate poisoning in hens

作　　者：王英鹏[1] 宋俊峰[1] 饶志仁[1] 牟丹蕾[2] 

机构地区：[1]第四军医大学全军神经科学研究所,西安710032 [2]唐都医院传染科

出　　处：《中华劳动卫生职业病杂志》2004年第1期19-21,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目(3970 0 1 2 2 )

摘　　要：目的　研究细胞凋亡机制在有机磷中毒后的动物迟发性神经病 (OPIDN)中所起的作用及其动态病理改变。方法　采用给母鸡一次性肌内注射三磷酸甲酯染毒的方法建立OPIDN动物模型 ,以染毒后 3、5、7、10、14、18d为时间点分别取材 ,以HE染色、尼氏法和原位末端标记法 (TUNEL)观察其第三腰髓 (L3 )的病理改变、神经元数量及细胞凋亡的变化。结果　母鸡于肌内注射三磷酸甲酯后第 9天前后出现进行性共济失调和肌无力等OPIDN的典型症状。HE染色示染毒后 5d后母鸡脊髓前角大神经元出现细胞核红色深染 ,至 18d消失 ;尼氏法染色示母鸡脊髓前角神经元数量呈进行性减少 [从 (82±4)个 /mm2 到 (66± 6)个 /mm2 ] ;TUNEL法示母鸡腰段脊髓TUNEL阳性细胞于染毒后 5d左右开始出现[(2 2± 2 )个 /mm2 ] ,以 7d[(2 7± 3 )个 /mm2 ]数量最多 ,18d消失。结论　在母鸡OPIDN发病过程中出现腰髓前角神经元细胞凋亡现象。ObjectiveTo investigate the role of neuron al apoptosis in organophosphorus poisoning-induced delayed neuropathy(OPIDN) a nd its dynamic pathological changes. MethodsTo establish OPIDN animal model,triorthocresyl phosphate(TOCP)was given to hens with a singl e dose(1 000 mg/kg,im).Changes of neuropathology,number of neurons and apoptotic cells in the third lumbar spinal cord were observed by HE,Nissl and TUNEL metho ds 3,5,7,10,14,18 days after injection. ResultsThe hens showed OPIDN typical signs(progressive ataxia and hypotonia) about 9 days after TOCP exposure.HE staining revealed dark red nucleus in neurons of anterior horn of lumbar spinal cord 5 days after exposure,but this phenomenon disappeared 18 d ays later.Nissl method showed that the number of neurons in anterior horn of spi nal cord decreased[from (82±4) cell/mm 2 to (66±6) cell/mm 2].TUNEL positi ve cells began to appear[(22±2) cell/mm 2] 5 days after TOCP exposure,and re ached the peak [(27±3) cell/mm 2] 7 days later,and disappeared 18 days l ater. ConclusionNeuronal apoptosis in anterior horn of s pinal cord of hens appeared in OPIDN,suggesting that cellular apoptosis may play an important role in the pathogenesis of OPIDN.

关 键 词：三磷酸甲酯中毒 母鸡 脊髓神经元 神经元凋亡 OPIDN 细胞凋亡 

分 类 号：R747.9[医药卫生—神经病学与精神病学]