ATP生物荧光肿瘤药敏检测技术在乳腺癌化疗中的应用价值  

Application Value of ATP Based Bioluminescence Tumor Chemoesensitivity Assay in the Chemotherapy of Breast Cancer

作　　者：陈国际[1] 傅军[1] 邵永孚[1] 张保宁[1] 徐兵河[1] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤医院肿瘤研究所,100021

出　　处：《实用癌症杂志》2004年第3期292-294,共3页The Practical Journal of Cancer

摘　　要：目的　探讨ATP生物荧光肿瘤药敏检测技术 (ATP TCA )在乳腺癌化疗中的临床意义及应用价值。方法　选用 10种常用乳腺癌化疗药物 ,应用ATP TCA对 40例乳腺癌改良根治术标本、淋巴结活检和胸腔积液标本进行药敏检测 ,评估肿瘤细胞对化疗药物的敏感性。结果　ATP TCA对乳腺癌标本的可评价率为 90 .0 % ,化疗药物对乳腺癌的杀伤作用具有较强的个体差异性 ,10种化疗药物的敏感性分别为 :氟尿嘧啶 ( 5 Fu) 3 3 .3 %、顺铂 (DDP) 3 7.5 %、环磷酰胺 (CTX ) 2 9.2 %、足叶已甙 (VP 16)16.7%、丝裂霉素 (MMC) 2 2 .0 %、表阿霉素 (EPI) 41.7%、诺维本 (NVB) 45 .8%、阿霉素 (ADM ) 41.7%、泰素 (PTX) 5 4.2 %、羟基喜树碱 (HCPT) 2 5 .0 %。初步研究表明ATP TCA体外检测结果与实际临床疗效具有良好的相关性。结论　ATP TCA是准确的、可靠的肿瘤药敏检测技术 ,其检测结果与临床实际疗效具有良好相关性 。Objective To Study the clinical value and application of ATP based Bioluminescence Tumor Chemosensitivity Assay(ATP-TCA) in the chemotherapy of breast cancer.Methods 10 kinds of chemotherapeutic drugs were applied and 40 breast cancer specimens from resection surgery,lymph node biopsy and pleural effusion were analyzed by ATP-TCA.Sensitivity of chemotherapeutic drugs was assessed.Results 36 of 40 specimens were evaluated with an overall evaluation rate of 90.0%.The assay results suggested that chemo-naive patients responded to chemotherapeutic drugs with individualized profiles.The sensitivity rate of 5-FU,DDP,CTX,VP-16,MMC,EPI,NVB,ADM,PTX and HCPT is 33.3%,37.5%,29.2%,16.7%,22.0%,41.7%,45.8%,41.7%,54.2% and 25.0%,respectively.Preliminary study results correlated well with clinical outcome.Conclusion ATP-TCA is a choice for the screening of chemotherapeutic drugs against breast cancer with excellent sensitivity and reliability.ATP-TCA assay results correlate well with clinical outcome,suggesting its clinical value in the management of individualized chemotherapy in breast cancer.

关 键 词：ATP生物荧光 肿瘤 药敏检测技术 乳腺癌 化疗 ATP-TCA 

分 类 号：R737.9[医药卫生—肿瘤]