川芎嗪对大鼠局灶性脑缺血后额叶、丘脑、小脑病理形态改变的影响  

Intervention Effect of Ligustrazine on Pathology of Frontal Lobes, Thalamuses, Cerebellums after Focal Ischemia in Rats

作　　者：楚冰[1] 邵国富[1] 周旭平[1] 

机构地区：[1]苏州大学附属第二医院神经内科,215000

出　　处：《神经疾病与精神卫生》2004年第4期250-251,共2页Journal of Neuroscience and Mental Health

摘　　要：目的　观察大鼠一侧大脑中动脉闭塞 (MCAO)后两侧额叶、丘脑、小脑细胞形态学变化及川芎嗪的干预作用。方法　采用线栓法建立大鼠一侧大脑中动脉闭塞模型 ,按实验需要将实验动物分成正常、单纯缺血及川芎嗪干预组 ;用HE染色和TUNEL法观察两侧额叶、丘脑、小脑MCAO后 6h、2 4h、1w细胞形态学变化。结果　同侧额叶、丘脑MCAO后 6h开始出现TUNEL阳性细胞 ,2 4h后明显增加 ,至 1周阳性细胞减少 (P <0 .0 1) ,而对侧丘脑、额叶及两侧小脑未见明显细胞形态学改变 ;干预组同侧额叶、丘脑凋亡阳性细胞数明显减少 (P<0 .0 5 )。结论　川芎嗪能增加MCAO后远隔部位的脑灌注 。Objective To observe the cell morphology of bilateral frontal lobes, thalamuses, cerebellums after unilateral MCAO and intervention effect of ligustrazine. Methods To establish a model of unilateral MCAO in rat according to Zea Longa’s method and divide animals into normal group, ischemic group and treatment group; To observe cell morphology at 6h, 24h, 1w variably after MCAO by TUNEL method and HE method. Results We find TUNEL positive cells in frontal lobe thalamus of the same hemisphere about 6h after MCAO, and increase more significantly at 24h, then decrease at 1w (P< 0.01). The TUNEL positive cells decrease in the group those received LZ compared with those without LZ (P< 0.05).Conclusions Ligustrazine can increase the perfusion of frontal lobes, thalamuses, cerebellums.

关 键 词：川芎嗪 大鼠 局灶性脑缺血 额叶 丘脑 小脑 病理学 中医药疗法 

分 类 号：R743.3[医药卫生—神经病学与精神病学]