High-Performance Liquid Chromatographic Method for Determination of Germacrone in Rat Plasma  

作　　者：HEHai-bing TANGXing CUIFu-de 

机构地区：[1]SchoolofPharmacy,ShenyangPharmaceuticalUniversity,Shenyang110016,China

出　　处：《Journal of Chinese Pharmaceutical Sciences》2004年第3期190-192,共3页中国药学（英文版）

摘　　要：Aim A reliable high-performance liquid chromatographic (HPLC) method wasdeveloped for determination of germacrone in rat plasma. Methods The plasma samples were treatedwith acetonitrile and analyzed by HPLC with UV detection at 244 nm. Results The limit of detectionwas 100 ng·mL^(-1) for germacrone in plasma and the linear range was 0.1004-15.06 μg·mL^(-1) inplasma. The RSD of intra-day and inter-day assay was 1.87% - 4.29% and 1.29% -5.15%, respectively.The recoveries of germacrone were over 95%. The endogenous substances in plasma did not show anyinterference in the analysis. Conclusion The method is accurate and convenient, and suitable forpharmacokinetic studies of germacrone in rats.Aim A reliable high-performance liquid chromatographic (HPLC) method was developed for determination of gennacrone in rat. plasma. Methods The plasma samples were treated with acetonitrile and analyzed by HPLC with UV detection at 244 nm. Results The limit of detection was 100 ng·mL^-1 for germacrone in plasma and the linear range was0.1004-15.06μg· mL^-1 in plasma. The RSD of intra-day and inter-day assay was 1.87%-4.29% and 1.29%-5.15%, respectively. The recoveries of germacrone were over 95%. The endogenous substances in plasma did not show any interference in the analysis. Conclusion The method is accurate and convenient, and suitable for pharmacokinetic studies of gennacrone in rats.

关 键 词：high-performance liquid chromatography GERMACRONE volatile oil of rhizomacurcumae 

分 类 号：R927.2[医药卫生—药学]