A Study of Monocyte Excretion of TNF- α and IL-6 and Monocyte Expression of HLA Class Ⅱ Antigen In Genital Herpes  

生殖器疱疹患者单核细胞分泌TNF-α、IL-6和HLA-Ⅱ类抗原表达的研究(英文)

作　　者：敖俊红[1] 杨蓉娅[1] 宋克敏[1] 周礼义[2] 陈兴平[2] 

机构地区：[1]北京军区总医院皮肤科,北京100700 [2]华中科技大学同济医学院附属同济医院皮肤科

出　　处：《Chinese Journal of Sexually Transmitted Infections》2004年第2期86-88,i002,共4页中华性传播感染杂志（英文版）

摘　　要：Objective: To study the role of monocytes in the pathogenesis of genital herpes. Methods: TNF- α and IL-6 levels in 27 cases of genital herpes were detected by enzyme linked immunosorbant assay (ELISA). HLA class Ⅱ antigen expression on monocytes were detected by an alkaline phosphatase anti-alkaline phosphatase method. Results: Compared with normal controls, levels of TNF- a and IL - 6 secreted by monocytes responding to LPS mitogen in vitro were significantly decreased [(3.13 ± 0.44ng/ml) vs (4.68 ± 0.54ng/ml), P<0.05 and (3.32 ± 1.06ng/ml) vs (6.46 ± 1.94ng/ ml), P<0.05, respectively]. HLA class Ⅱ antigen expression on monocytes in the genital herpes group was also significantly decreased [HLA-DR (67.48% ± 1.51%) vs (81.03% ± 1.32%), P<0.01 and HLA-DQ (29.54% ± 1.15%) vs (37.63% ± 1.79%), P <0.01 respectively]. Conclusion: These findings suggest that the decreased monocyte function may contribute to the pathogenesis of genital herpes. Augmenting or inducing monocyte function may be important in the prevention, treatment, and reduction of genital herpes cases.目的研究单核细胞在生殖器疱疹发病机理中的作用。方法采用ELISA法,检测27例生殖疱疹患者外周血单核细胞经内毒素(LPS)诱生后培养上清中TNF-α、IL-6水平;采用APAAP 法,检测生殖器疱疹患者外周血单核细胞HLA-Ⅱ类抗原表达水平。结果生殖器疱疹患者外周血单核细胞分泌TNF-α、IL-6水平分别为3.13±0.44ng/ml和3.32±1.06ng/ml,较正常对照组[分别为(4.68±0.54ng/m1)和(6.46±1.94ng/m1)]明显降低,差异有显著性意义(P<0.05); 生殖器疱疹患者外周血单核细胞HLA-DR、HLA-DQ表达水平分别为67.48%±1.51%和29.54 %±1.15%,较正常对照组[分别为(81.03%±1.32%)和(37.63%±1.79%)]明显降低,差异有显著性意义(P<0.01)。结论单核细胞功能降低与生殖器疱疹发病有关,提高单核细胞功能, 对生殖器疱疹的防治,减少复发率有一定意义。

关 键 词：Genital herpes MONOCYTE Tumor necrosis factor- α INTERLEUKIN-6 ULA class Ⅱ antigen 

分 类 号：R752.1[医药卫生—皮肤病学与性病学]