脐血树突状细胞介导的食管癌疫苗实验研究  

Experimental study of cancer vaccine prepared by fusions of esophageal carcinoma cell line with dendritic cell derived from cord blood

作　　者：郭光华[1] 陈素钻[1] 张娟[1] 罗丽莉[1] 苏中静[1] 董红梅[1] 徐虹[1] 吴利标[1] 

机构地区：[1]汕头大学医学院第一附属医院消化内科,汕头515041

出　　处：《中国实用内科杂志》2004年第11期661-662,共2页Chinese Journal of Practical Internal Medicine

基　　金：广东省自然科学基金资助 (编号 0 2 12 2 8)

摘　　要：目的　用杂交瘤技术制备脐血树突状细胞 (DC)和食管癌细胞融合疫苗 ,分析其生物学特征及体外诱导特异性免疫应答的能力。方法　 2 0 0 2 - 0 12 0 0 3- 0 3汕头大学医院第一附属医院分离脐血CD3 4 + 干细胞并诱导扩增为成熟DC ,并与EC10 9细胞融合 ,免疫磁珠法筛选EC10 9 DC ;流式细胞术鉴定疫苗表型 ;绘制疫苗生长曲线 ;MTT法测定瘤苗诱导淋巴细胞增殖能力及体外特异性免疫应答能力。结果　疫苗可体外生长 ,高表达CD80 、CD83 和CD86,并能有效刺激淋巴细胞增殖反应 ,体外诱导细胞毒T细胞 (CTL)对EC10 9的杀伤活性显著高于对照组 (P <0 0 5 )。结论　EC10 9 DC具备了刺激免疫细胞活化的分子表型 。Objective To establish the cancer vaccine (EC109-DC) prepared by fusions of esophageal carcinoma cell line with dendritic cell derived from cord blood and study the biological characteristics and antitumor immunity.Methods CD 34 + hematopoietic stem cells were isolated from cord blood using CD 34 + Progenitor Cell Isolation Kit by magnetic cell sorting system (MACS);expended the cells as DC,and then fused with EC109 by PEG-3600;selected the fusion cells by MACS;observed the phenotypes and proliferation by flow cytometry and technique of cell culture in vitro. Lymphocytes proliferation reaction and the CTL cytotoxicity were examined with MTT.Results EC109-DC could proliferate slowly in vitro and highly expressed CD80,CD83 and CD86.Lymphocytes proliferation reaction and the specific cytotoxicity against EC109 induced by EC109-DC cells were significantly higher than the control groups (P<0.05).Conclusion EC109-DC vaccine acquires the immuno-stimulating phenotype and can significantly stimulate the lymphocytes proliferation reaction, and the CTL activity.

关 键 词：脐血 树突状细胞 食管癌 疫苗 实验 

分 类 号：R735.1[医药卫生—肿瘤]