梧桐花粉致敏大鼠动物模型的建立及特异性抗体检测  

Animal Models of Allergic Diseases Induced by Platanus acerifolia Pollen and Measurements of Specific Antibodies

作　　者：陈小东[1] 陈臻[1] 张尚权[2] 吴芝莉[2] 王逸宏 李逸平[2] 

机构地区：[1]复旦大学附属华山医院呼吸科,上海200040 [2]中国科学院上海生命科学研究院生物化学与细胞生物学研究所,上海200031

出　　处：《细胞生物学杂志》2004年第5期523-525,共3页Chinese Journal of Cell Biology

摘　　要：用梧桐花粉浸液作为过敏原,经皮下多点注射对实验动物进行免疫,用雾化的梧桐花粉浸液进行激发,建立了变态反应性疾病的实验动物模型。随后通过ELISA、Western印迹等方法对其血清中的梧桐抗原特异性IgG、IgE进行检测。结果显示有特异性IgG、IgE抗体的存在。Allergic diseases of rats were induced by allergen extracted from Platanus acerifolia pollen. Firstly, rats were injected intraperitoneally and subcutaneously with the mixture of allergen and adjuvant. Subsequently, these rats were challenged with aerosolized allergen. After the last inspiration, rats were killed and their serums were separated. Specific IgE and IgG in serums were measured by ELISA or Western blotting. The results revealed the existence of specific IgE and IgG.

关 键 词：梧桐花粉 过敏原 实验动物 大鼠 动物模型 变态反应 IGG IGE 免疫球蛋白 

分 类 号：R-332[医药卫生] R446.6