非小细胞肺癌病理类型及临床分期与血液端粒酶活性相关性研究  

Correlation of pathological types and clinical stages of NSCLC with the blood telomerase activity

作　　者：胡坚[1] 孙鹂[2] 李任远[1] 倪一鸣[1] 

机构地区：[1]浙江大学医学院附属第一医院心胸外科,杭州310003 [2]杭州师范学院医学院科研实验室

出　　处：《中华实验外科杂志》2004年第10期1257-1258,共2页Chinese Journal of Experimental Surgery

基　　金：浙江省教育厅资助项目 (1 9990 0 88);浙江省自然科学基金资助项目(M30 3778)

摘　　要：目的　分析非小细胞肺癌患者不同病理类型及临床分期血液端粒酶活性表达的差异。方法　采用端粒重复序列扩增 (TRAP) 酶联免疫吸附法 (ELISA)检测 3 8例肺癌患者血液端粒酶活性 ,并且与 2 5例肺部良性疾病进行对照 ,其结果予以定性及半定量。结果　肺腺癌患者术中及术后血液端粒酶活性分别为 0 .14 9± 0 .0 18、0 .116± 0 .0 19,明显高于术前血液端粒酶活性0 .0 72± 0 .0 12 ,差异有显著性 (P <0 .0 1,P <0 .0 5 ) ;术后肺腺癌患者血液端粒酶为 0 .116± 0 .0 19,鳞癌为 0 .0 62± 0 .0 0 8,差异有非常显著性 (P <0 .0 1) ;术后ⅢA ⅢB期患者血液端粒酶活性为0 .13 1± 0 .0 2 3 ,Ⅰ Ⅱ期为 0 .0 65± 0 .0 11,差异有非常显著性 (P <0 .0 1)。Objective To investigate the expression of blood telomerase activity in different pathological types and clinical stages of NSCLC.Methods PCR-TRAP-ELISA was used to measure telomerase activity in 38 NSCLC patients and 25 inpatients with benign lung diseases.Results Blood telomerase activity of the patients with adenocarcinoma during and after operation was 0.149±0.018 and 0.116±0.019 respectively,significantly higher than that before operation (P< 0.01, P< 0.05). In adenocarcinoma,blood telomerase activity after operation was significantly higher than that in squamous carcinoma (P< 0.01). After operation,blood telomerase activity in ⅢA-ⅢB stage was 0.131± 0.023, significantly higher than that in Ⅰ-Ⅱ stage (P< 0.01). Conclusion Difference in the blood telomerase activity in pathological types and clinical stages is hopeful to make prognostic judgment of NSCLC.

关 键 词：肺癌 病理学 端粒酶 临床分期 病理类型 

分 类 号：R734.2[医药卫生—肿瘤]