脑缺血再灌注损伤后神经细胞巢蛋白和干细胞因子基因表达  

The gene expressions of nestin and stem cell factor in neurons after cerebral ischemia-reperfusion in rats

作　　者：郑青立 龚薇薇[2] 王玲[2] 孙成云[2] 郭云良[2] 

机构地区：[1]山东省青岛疗养院门诊部,青岛266071 [2]青岛大学医学院脑血管病研究所

出　　处：《中华物理医学与康复杂志》2004年第10期589-591,共3页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：山东省自然科学基金项目 (No.Y2 0 0 1C0 4 );山东省卫生厅资助项目 (No .2 0 0 1CA1DRB1 )

摘　　要：目的　研究大鼠脑缺血再灌注损伤后神经细胞巢蛋白 (nestin)和干细胞因子 (SCF)基因表达的变化。方法　成年健康雌性SD大鼠 3 6只 ,以线栓法建立大脑中动脉缺血再灌注模型 ,随机分为缺血 1.5h再灌注 2h、6h、12h、2 4h、2d、3d、7d、14d组和假手术组 ,每组 4只。应用原位杂交技术检测脑缺血再灌注后脑组织nestin和SCFmRNA的表达。结果　假手术组皮质、纹状体和室旁区nestinmRNA表达很弱。缺血再灌注后nestinmRNA表达 ,皮质除 2h以外、纹状体除 2h、6h以外、室旁区除 2h、6h、14d以外各时间点均明显高于假手术组。假手术组皮质、纹状体和室旁区SCF表达很弱。缺血再灌注后SCFmRNA表达 ,皮质除 2h、6h、12h以外、纹状体除 2h、6h以外、室旁区除 2h、14d以外各时间点均明显高于假手术组。结论　脑缺血再灌注后SCFmRNA表达可能具有促进神经干细胞增殖作用。Objective To study the gene expressions of nestin and stem cell factor(SCF)in neurons after ischemia-reperfusion injury in rat brain. Methods Thirty-six adult female rats were subject to left middle cerebral artery occlusion for 1.5h and different hours of reperfusion. In site hybridization was used to examine the expression of nestin and SCF mRNA in the rats subjected to 2h, 6h, 12h, 24h, 2d, 3d, 7d, 14d of reperfusion and sham-operation group (n=4). Results (1) Nestin expression in cortex, striatum and extraventricular zone was weak in the sham-operation group, and increased markedly in the ischemic hemisphere compared with sham-operation group except of reperfusion 2h in cortex, 2h, 6h in striatum, 2h, 6h and 14d in extraventricular zone. (2)SCF expression in cortex, striatum and extraventricular zone was weak in the sham-operation group, and increased markedly in the ischemic hemisphere compared with sham-operation group except of reperfusion 2h, 6h, 12h in cortex, 2h, 6h in striatum, 2h and 14d in extraventricular zone. Conclusion It is suggested that SCF expression might enhance the proliferation of neural stem cells following ischemia-reperfusion in rats.

关 键 词：mRNA表达 手术 纹状体 脑缺血再灌注损伤 干细胞因子 皮质 神经细胞 SCF 巢蛋白 雌性 

分 类 号：R743[医药卫生—神经病学与精神病学] R338[医药卫生—临床医学]