海南霉素对山羊瘤胃内肽的保护作用  

Protective effects of hainanmysin on concentration of rumen peptides in goats

作　　者：沈赞明[1] 陈杰[1] 任明强[1] 

机构地区：[1]南京农业大学农业部动物生理生化重点开放实验室,江苏南京210095

出　　处：《南京农业大学学报》2004年第4期73-76,共4页Journal of Nanjing Agricultural University

基　　金：江苏省自然科学基金 (BK990 5 8);农业部重点资助项目 (95牧 0 2 0 1)

摘　　要：对 3只成年公山羊采用自身对照法 ,分 4期 ,每期 10d ,依次每天 2次 [8:0 0和 2 0 :0 0 ]经瘤胃瘘管灌注海南霉素 (HM) ,剂量分别为 0 (对照 )、 0 0 0 2 (HM1)、 0 0 1(HM2 )和 0 0 5mg·kg -1·d-1(HM3 )。实验结果表明 ,3个HM组的山羊瘤胃内肽浓度持续上升并高于对照 ,第 10小时比对照组分别上升了 6 0 4 5 % (P <0 0 5 )、 6 7 15 % (P <0 0 5 )和5 4 72 % (P <0 0 5 ) ;瘤胃内肽平均浓度显著提高 ;HM1和HM2 组瘤胃可溶性蛋白浓度增加 (P <0 0 5 )。在分别添加了0 2 5、 0 5 0、 1 0 0、 2 5 0和 5 0 0mg·L-1的HM条件下 ,体外厌氧培养瘤胃微生物 ,并研究其对培养液中蛋白酶、肽酶活性的影响。结果发现 ,5种剂量的HM均显著抑制瘤胃肽酶活性 (P <0 0 5 ) ,并呈现剂量效应。其中 4种剂量 (0 2 5、0 5 0、 1 0 0和 2 5 0mg·L-1)显著抑制瘤胃蛋白酶活性 (P <0 0 5 ) ,高剂量 (5 0 0mg·L-1)的作用不显著。以上结果表明 :海南霉素具有保护瘤胃肽和可溶性蛋白的作用 ,该作用是海南霉素抑制瘤胃蛋白酶、肽酶活性的结果。Three doses of hainanmysin(0.002 mg·kg -1·d -1 for HM 1,0.01 mg·kg -1·d -1 for HM 2,0.05 mg·kg -1·d -1 for HM 3)were infused into rumen of 3 male goats via rumen fistula by using self-control method.The study included 4 periods,each period lasted for 10 days.The infusion was performed twice(8:00 and 20:00)every day.The result showed that,compared with the control group,the ruminal peptied concentration in 3 HM groups(HM s)increased from 1st hour to 10th hour after morning feeding,and at the 10 th hour the ruminal peptide concentration of HM s increased by 60.45%(P<0.05),67.15%(P<0.05)and 54.72%(P<0.05),respectively.The average ruminal peptide concentration was also significantly higher in HM s than that in the control(P<0.05).The ruminal concentration of soluble protein was higher in both HM 1 and HM 2,but not in HM 3.In vitro the rumen microbes were anaerobically incubated after 5 doses of HM(0.25,0.50,1.00,2.50 and 5.00 mg·L -1)to determine the activities of peptidase and proteinase.The result revealed that 5 doses of HM reduced peptidase activity significantly(P<0.05)in a dose-dependent manner.And ,HM at doses of 0.25,0.50,1.00,and 2.50 mg·L -1 but not dose of 5.00 mg·L -1 inhibited the proteinase activity significantly(P<0.05).These results showed that HM protect ruminal peptides and protein by inhibiting the peptidase and proteinase activities in the rumin.

关 键 词：海南霉素 瘤胃肽 瘤胃蛋白质 肽酶活性 蛋白酶活性 

分 类 号：S852.21[农业科学—基础兽医学]