逆境下转BADH基因小麦甜菜碱醛脱氢酶活性表达与甜菜碱积累  

Betaine Accumulation and Aldehyde Betaine Dehydrogenase Enzyme Activity for Transgenic Wheat under Stress Condition

作　　者：张艳敏[1] 丁占生[1] 温之雨[1] 蒋春志[2] 李辉[2] 霍云谦[1] 朱至清[3] 陈受宜[4] 郭北海[1] 

机构地区：[1]河北省农林科学院遗传生理研究所 [2]河北省农科院粮油作物所 [3]中国科学院植物所 [4]中国科学院遗传发育研究所

出　　处：《华北农学报》2003年第F09期36-39,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家863计划(2001AA212121):植物转基因其产业化专项(J99-B-010)资助

摘　　要：对盐、旱逆境下转BADH基因小麦品系99T6的生长发育、甜菜碱醛脱氢酶活性及甜菜碱积累等进行了研究分析,结果表明,转BADH基因株系在盐逆境下具有明显的生长优势,耐盐能力达到100mmol/L;电导率和质膜相对透性表明转基因株系抗膜损伤能力增强;甜菜碱醛脱氢酶活性的增强和甜菜碱积累的增加,说明转基因株系的盐旱逆境抗性是通过甜菜碱积累这一长期、持久的方式解除渗透胁迫,而不是通过脯氨酸积累这种临时的应急反应;以甜菜碱作为靶标性状采用基因工程方法提高植物盐旱耐性是可行的。Assay for salt tolerance and drought resistance of transgenic wheat with BADH cDNA by biolistic method were conducted under simulated salt/drought stress condition. The results indicated that the transgenic wheat has many obvious advantages over its received plants, such as the more vigorous development of seedlings, the well developed root system and the greater root activity under salt/drought stress condition, as well as the improved plasma membrane protection of excised leaf. The betaine aldehyde dehydrogenase enzyme activity under different salt concentration is also higher, and more betaine accumulation under drought condition, than its received plant. This indicated that the introduction of BADH gene into wheat may affect a series of physiological reaction to adapt stress condition, this may be one of the reasons for its higher salt/drought tolerance. The way to increase crop stress tolerance/resistance through transgenic method may be effective.

关 键 词：转BADH基因 小麦 甜菜碱 抗盐旱耐性 醛脱氢酶 活性表达 胁迫条件 

分 类 号：S512.103.53[农业科学—作物学]