c-myc、bcl-2基因表达和蝌蚪提取液抗早幼粒细胞白血病作用的研究  

Expression of c-myc,bcl-2 and the effects of tadpole extract on human promyelocytic leukemia cells

作　　者：杨红梅[1] 尤笑迎[2] 王黎[1] 陈洁[1] 

机构地区：[1]河南职工医学院病生教研室,郑州450003 [2]郑州市第五人民医院

出　　处：《江苏医药》2003年第10期745-747,共3页Jiangsu Medical Journal

摘　　要：目的　研究蝌蚪提取液 (T871)抗早幼粒细胞白血病 (APL)的作用及其过程中c myc、bcl 2癌基因表达的变化。方法　利用细胞生长曲线、形态学观察、流式细胞仪、TUNEL法及原位mRNA杂交和完整细胞原位斑点印迹技术 ,观察T871抗人APL细胞系 (HL 6 0 )细胞的作用及其过程中c myc、bcl 2癌基因表达的变化。结果　T871能抑制HL 6 0细胞增殖 ,并出现细胞凋亡的形态学特征 ,流式细胞仪及TUNEL法检测结果显示凋亡细胞百分数比对照组显著增加 ,并伴有癌基因c myc、bcl 2mRNA表达的下调。 结论　T871可抑制HL 6 0细胞的增殖同时诱导其凋亡 ,且c myc、bclObjective To investigate the function of antileukemia by tadpole extract(T871)and expression of c-myc,bcl-2 in the process.Methods We studied the effects of T871 on proliferation and apoptosis of human promyelocytic leukemia cell line(HL-60)cells by cell growth stage,cytomorphological observation,flow cytometry analysis and TUNEL method.We also examined the oncogene(c-myc?bcl-2)expression during the induction of apoptosis in T871 treated HL-60 cells by in situ hybridization and intact cell mRNA dot blot techniques.Results T871 inhibited the proliferation of HL-60 cells.T871 induced HL-60 cells to apoptosis as evidenced by:(1)characteristic morphology of apoptosis.(2)The flow cytometry analysis and TUNEL methods analysis showed an increase in the number of apototic HL-60 cells increased.The apoptosis of HL-60 cells was accompanied by down-regulation of c-myc,bcl-2 oncogene expression.Conclusion Tadpole extract may induce HL-60 cells to apoptosis and oncogene c-myc,bcl-2 may involve in the apoptotic course.

关 键 词：C-MYC 早幼粒细胞白血病 bcl-2 癌基因表达 TUNEL法 HL-60细胞 流式细胞仪 RNA 蝌蚪 形态学特征 

分 类 号：R733[医药卫生—肿瘤] R735[医药卫生—临床医学]