间接分光光度法测定抗坏血酸含量  

Determination of Ascorbic Acid by Indirect Spectrophotometry

作　　者：徐黔江[1] 许乾丽[2] 

机构地区：[1]贵阳医学院物理化学教研室,贵州贵阳550004 [2]贵州省理化测试中心,贵州贵阳550002

出　　处：《贵阳医学院学报》2001年第2期129-130,共2页Journal of Guiyang Medical College

摘　　要：目的 :测定药物中的抗坏血酸含量。方法 :利用抗坏血酸对铁 (Ⅲ ) - 4- (2 -苯并噻唑偶氮 )邻苯二酚 -乳化剂OP络合物的还原作用 ,生成铁 (Ⅱ ) - 4- (2 -苯并噻唑偶氮 )邻苯二酚 -乳化剂OP络合物 ,使用分光光度法间接测定抗坏血酸。结果 :测定了抗坏血酸片和Vc注射液的含量 ,并与碘量法比较 ,同时还测定了标准回收率。结论 :此法方便快捷 ,测定含量与碘量法一致 。Objective:To analyse the content of ascorbic acid in medicine.Methods:Ascorbic acid was determined by spectrophotometric method based on the formation of iron(Ⅱ)-4-(2-benzothiazolylazo) pyrocatechol-OP complex which was the product of the redox reaction between ascorbic acid and iron (Ⅲ)-4-(2-benzothiazolylazo) pyrocatechol-OP complex. The proposed method is rapid and satisfactory.Results:The content of Ascorbic Acid in medicines have been determined and compared with the results of iodine method.Conclution:The proposed method is rapid and satisfaction and the results is consistence with the iodine method.

关 键 词：抗坏血酸 分光光度法 铁(Ⅲ)-4-(2-苯并噻唑偶氮)邻苯二酚 

分 类 号：R917.7[医药卫生—药物分析学]