人胃癌细胞系BGC—823不同转移能力的细胞克隆间的细胞微丝结构特征研究  

COMPARATIVE STUDY ON THE STRUCTURAL CHARACTERISTICS OF MICROFILAMENTS IN CELLS WITH DIFFERENT METASTATIC ABILITY CLONED FROM HUMAN GASTRIC CANCER CELL LINE BGC-823

作　　者：陆锐 柯杨 路桂荣 宁涛 刘伟莉 廖静 王冰 鄂征 

机构地区：[1]北京市肿瘤防治研究所遗传室,北京100034

出　　处：《解剖学报》1993年第4期386-390,共5页Acta Anatomica Sinica

摘　　要：用单细胞培养法,从人胃癌细胞系BGC-823中分离出10个克隆。通过裸鼠致瘤和转移检测,进一步筛选出具有较高转移性的C_1、C_6和低转移的C_5、C_8等4个细胞株。它们的生长能力虽无明显差异,但超微结构显示C_5和C_8细胞表面微绒毛少,而且长度不一,微丝长而粗,排列规则,组装程度较好;C_1和C_6细胞微绒毛多而密集,长度均一,微丝短而粗,呈点状或玫瑰状小体,组装程度差。此外,本研究显示肿瘤细胞内的微丝骨架组装状态,与转移能力似呈负相关。Ten cell strains were obtained from the human gastric cancer cell line BGC-823 by using the method of single cell cloning. Through inoculation of these cell strains in nude mice and subsequent observation of their metastatic capability, four cell lines, C_1, C_6 with high, and C_5, C_8 with low metastatic capability were selected out; and their biological and morphological properties were studied. It was demonstrated that all of them did not show apparent difference in growth ability, but morphologically, the mictovilli of C_5 and C_8 cells were scarce and short; and their microfilaments were thick, straight and well organized. Whereas the microvilli of C_1 and C_6 strains were long and abundant comparatively, but their microfilaments were poorly organized. On the basis of present observation,we suggested that the organization of microfilaments in cancer cells appeared to bear reversed relationship with their metastatic capability.

关 键 词：BGC-823 细胞系 癌细胞转移 微丝 

分 类 号：R730.21[医药卫生—肿瘤]