蟾蜍灵对人胃癌细胞系MGc-803的细胞毒作用  

Studies on the Cytotoxicity of Bufalin on Human Gastric Cancer Cell MGc-803 in vitro

作　　者：陈小义[1] 徐瑞成[1] 陈莉[1] 钱进[2] 

机构地区：[1]中国人民武装警察部队医学院,天津300162 [2]天津药物研究院

出　　处：《中草药》2000年第12期920-922,共3页Chinese Traditional and Herbal Drugs

摘　　要：蟾蜍灵(bufalin)是我国传统中药蟾酥中蟾毒的主要成分之 一，研究其抑癌作用及机制具有重要意义。采用台盼蓝染色法、噻唑蓝还原法和光镜观察bu falin对人低分化胃腺癌细胞系MGc-803的生长抑制作用。结果显示，0.01 μmol/L及以上 浓度bufalin对MGc-803细胞具有显著的抑制作用，IC50值约为0.1 μmol/L。bufal in抑制MGc-803生长的机制是致使细胞核染色质凝缩、碎裂，DNA损伤，胞浆RNA含量下降， 导致细胞死亡。To study the cytotoxic activity of bufalin, one of the components of bufadienolides in the TCM “Chansu”, and its mechanism of act ion. Human gastric cancer cell line MGc-803 was used as target cells. Inhibition of t umor cell growth was assessed by trypan blue exclusion and thiazole blue absorba nce and light microscope examination. Result showed that bufalin at concentrati ons of 0.01 μ mol/L and above could remarkably inhibit the growth of MGc-803 t um or cell, with IC50 of about 0.1 μ mol/L. Further study showed that its mechanism of action involved necrotic changes of nuclear chromatin and fragmentati on of chromosomal DNA leading to cell death.

关 键 词：蟾蜍灵 胃癌 肿瘤细胞系 MGC-803 细胞毒作用 中药制剂 

分 类 号：R273.52[医药卫生—中西医结合]