急性髓系白血病Th1/Th2细胞因子和T细胞亚群的变化及其临床意义  

Clinical value of Th1/Th2 cytokines level and the ratio of T lymphocyte subsets in patients with acute myeloblastic leukemia

作　　者：薛燕[1] 尤胜国[2] 葛薇[2] 马双[2] 

机构地区：[1]东南大学医学院附属徐州医院血液科,江苏徐州221009 [2]中国医学科学院血液学研究所免疫学实验室,天津300020

出　　处：《东南大学学报（医学版）》2004年第5期307-310,共4页Journal of Southeast University(Medical Science Edition)

摘　　要：目的 :探讨急性髓系白血病 (AML)患者化疗前后Th1 Th2类细胞因子水平、CD4+ CD8+ T细胞亚群比例的变化及其临床意义。方法 :采集AML患者的外周及骨髓血 ,以流式细胞仪检测单个核细胞免疫表型 ,以酶联免疫吸附试验检测血清和培养上清中IL 4、IFN γ水平 ,以放射免疫法检测IL 2水平。结果 :治疗前患者血清IL 4水平明显增高 ,而IFN γ水平则相反 ,部分高危患者缓解后IL 4水平仍较高 ,并且CD4+ CD8+ T细胞比例显著倒置。结论 :AML患者存在Th1 Th2类细胞因子水平及CD4+ CD8+ T细胞亚群比例的异常 ,有预后价值。Objective to explore whether AML patients have imbalanced cytokine levels and abnormal ratio of T lymphocyte subsets and their clinical value. Methods Peripheral blood and bone marrow were collected from AML patients during different periods. PBMNCs were detected by flowcytometry. The purified T lymphocytes were cultured and their supernatant was collected on different days.ELISA and RIA were used to measure the IL、4、IFN、γ and IL、2 level,respectively. Results The IL、4 levels in serum of pretreated AML cases were higher than those of the controls,while the IFN、γ levels on the contrary. IL、4 levels of high risk cases were found to be significantly higher than that of other cases, while IFN、γ levels much lower than the latter. The ratios of CD4+/CD8+ T lymphocyte subsets in high risk cases were much lower than that of the other cases. The duration of CR closely related to the ratio of CD4+/CD8+ T subsets(r=0.835、3,linear regression equation:y= 1.068+0.017x). Conclusion The imbalanced cytokine levels of Th1/Th2 subsets and reversed ratios of CD4+/CD8+ T lymphocyte subsets occurred in AML patients,which is of prognostic value.

关 键 词：急性髓系白血病 TH1/TH2细胞因子 T细胞亚群 免疫异常 预后 

分 类 号：R733.71[医药卫生—肿瘤]