SELDI蛋白质芯片技术在肿瘤蛋白质组学研究中的应用  

Application of SELDI proteinchip technique to the research of tumors' proteomics

作　　者：伍健[1] 黄培林[1] 

机构地区：[1]东南大学基础医学院病理学与病理生理学系,江苏南京210009

出　　处：《东南大学学报（医学版）》2004年第5期347-350,共4页Journal of Southeast University(Medical Science Edition)

摘　　要：SELDI蛋白质芯片技术是结合色谱学 层析原理和SELDI TOF MS技术制成的新一代生物芯片 ,是目前肿瘤蛋白质组学研究的重要工具之一。它的优点是能洞察肿瘤细胞、组织和生物体蛋白质含量的细微变化 ,且能精确分析某一特定蛋白质的理化性质。作者就SELDI蛋白质芯片联合二维电泳、激光捕获显微切割等技术在肿瘤蛋白质组学研究中所应采取的策略 ,SELDI蛋白质芯片在肿瘤蛋白质组学研究中的临床应用如筛选肿瘤标志蛋白、预测肿瘤转移和预后等作一综述。SELDI proteinchip technique, which combines the chromatography principle and SELDI、TOF、MS technique, is the newest biochip, and one of the important tools in the research of the tumors' proteomics. It can identify the subtle changes of the proteinic contents in the tumor's cells、tissues and bodies. Moreover, it can precisely analyse the chemical and physical qualities of a certain protein. This review retrospects the strategies in the research of tumors' proteomic of the proteinchip when combined with other techniques,like 2、D PAGE and LCM, etc,as well as the clinical application of the SELDI proteinchip to the research of tumors' proteomic such as identifying the tumors' proteinic marks and forecasting the metastasis and prognoses.

关 键 词：SELDI蛋白质芯片 蛋白质组学 蛋白质分离 生殖系统肿瘤 消化系统肿瘤 

分 类 号：R730[医药卫生—肿瘤]