趋化因子受体CXCR3在胃癌中的表达  

Expression of Chemokine Receptor CXCR3 in Gastric Cancer

作　　者：陈瑜[1] 张德新[1] 郭长存[1] 乔泰东[1] 樊代明[1] 吴开春[1] 

机构地区：[1]第四军医大学西京医院消化内科,710032

出　　处：《胃肠病学》2004年第5期266-269,共4页Chinese Journal of Gastroenterology

基　　金：国家自然科学基金(No.30225039和No.30130260);全军十五重点科研基金(No.01Z087)资助

摘　　要：背景近年来,趋化因子及其受体在肿瘤转移中的作用引起了重视,但有关趋化因子受体CXCR3在胃癌中的表达尚无报道。目的检测胃癌组织和细胞系中CXCR3在mRNA和蛋白水平的表达,为进一步研究其在胃癌转移中的作用奠定基础。方法应用逆转录聚合酶链反应(RT鄄PCR)检测胃癌细胞系CXCR3mRNA的表达,蛋白质印迹法(Westernblotting)检测胃癌组织和细胞系CXCR3蛋白的表达,流式细胞仪检测胃癌细胞系表面CXCR3的表达。结果在所检测的胃癌组织中,CXCR3蛋白的表达量明显高于相应癌旁组织。所检测的胃癌细胞系均有CXCR3mRNA和CXCR3蛋白表达,但仅KATOⅢ细胞表面CXCR3的表达较明显。永生化胃上皮细胞系GES鄄1的CXCR3蛋白表达量明显低于胃癌细胞系。结论胃癌细胞系有CXCR3表达,胃癌组织中CXCR3的表达高于癌旁组织。Background: The roles of chemokines and their receptors in tumor metastasis have received much attention of the investigators in recent years; however, the expression of chemokine receptor CXCR3 in gastric cancer is unknown. Aims: To investigate the expression of CXCR3 in gastric cancerous tissues and gastric cancer cell lines at mRNA and protein levels, and to pave the way for further investigating the role of CXCR3 in metastasis of gastric cancer. Methods: The mRNA expression of CXCR3 in gastric cancer cell lines was measured by reverse transcriptase polymerase chain reaction (RT-PCR), the protein expression of CXCR3 in gastric cancerous tissues and gastric cancer cell lines were then explored by Western blotting, and the expression of CXCR3 on the surface of gastric cancer cell lines was analyzed by flow cyto-metry. Results: In the tested gastric cancerous tissues, the protein expression of CXCR3 was obviously higher than that in the corresponding paracancerous tissues. CXCR3 expression at mRNA and protein levels could be detected in all tested gastric cancer cell lines, but its expression on cells surface was weak except that on the surface of KATOⅢ cells. The protein expression of CXCR3 in immortalized gastric epithelial cell line GES-1 was significantly lower than those in gastric cancer cell lines. Conclusions: CXCR3 is expressed in gastric cancer cell lines, and it is also present in gastric cancerous tissues with level higher than that in the paracancerous tissues.

关 键 词：趋化因子受体 CXCR3 胃癌 表达 

分 类 号：R735.2[医药卫生—肿瘤]