Reversal of 5-flouroucial resistance by adenovirus-mediated transfer of wild-type p53 gene in multidrug-resiatant human colon carcinoma LoVo/5-FU cells  

作　　者：Zhi-WeiYu PengZhao MingLiu Xin-ShuDong JiTao Xue-QinYao Xin-ttuaYin YuLi Song-BinFu 

机构地区：[1]DepartmentofAbdominalSurgery,theThirdAffiliatedHospitalofHarbinMedicalUniversity,Harbin150040,HeilongjiangProvince,China [2]DepartmentofGeneralSurgery,ThefifthHospitalofHarbinMedicalUniversity,Harbin150040,HeilongjiangProvince,China [3]DepartmentofGeneralSurgery,NanfangHospital,Guangzhou510515,GuangdongProvince,China [4]DepartmentofGeriatrics,theSecondAffiliatedHospitalofHarbinMedicalUniversity,Harbin150096,HeilongjiangProvinece,China [5]DepartmentofMolecularBiology,HarbinMedicalUniversity,Harbin150057,HeilongjiangProvince,China

出　　处：《World Journal of Gastroenterology》2004年第13期1979-1983,共5页世界胃肠病学杂志（英文版）

摘　　要：AIM: To observe the reversal effects of wide-type p53 gene on multi-drug resistance to 5-FU (LOVO/5-FU).METHODS: After treatment with Ad-p53, LOVO/5-FU sensitivity to 5-Fu was investigated using tetrazolium dye assay. Multidrug resistance gene-1 (MDR1) gene expression was assayed by semi-quantitative reverse transcriptionpolymerase chain reaction and the expression of p53 protein was examined by Western blotting.RESULTS: The reversal activity after treatment with wide type p53 gene was increased up to 4.982 fold at 48 h. The expression of MDR1 gene decreased significantly after treatment with wide-type p53 gene, and the expression of p53 protein lasted for about 5 d, with a peak at 48 h, and began to decrease at 72 h.CONCLUSION: Wide-type p53 gene has a remarkable reversal activity for the high expression of MDR1 gene in colorectal cancers. The reversal effects seem to be in a time dependent manner. It might have good prospects in clinical application.AIM:To observe the reversal effects of wide-type p53 gene on multi-drug resistance to 5-FU (LOVO/5-FU). METHODS:After treatment with Ad-p53,LOVO/5-FU sensitivity to 5-Fu was investigated using tetrazolium dye assay.Multidrug resistance gene-1 (MDR1) gene expression was assayed by semi-quantitative reverse transcription- polymerase chain reaction and the expression of p53 protein was examined by Western blotting. RESULTS:The reversal activity after treatment with wide- type p53 gene was increased up to 4.982 fold at 48 h.The expression of MDR1 gene decreased significantly after treatment with wide-type p53 gene,and the expression of p53 protein lasted for about 5 d,with a peak at 48 h,and began to decrease at 72 h. CONCLUSION:Wide-type p53 gene has a remarkable reversal activity for the high expression of MDR1 gene in colorectal cancers.The reversal effects seem to be in a time dependent manner.It might have good prospects in clinical application.

关 键 词：反向作用 5-氟 抵抗力 腺病毒 调节作用 基因表达 野生型 P53基因 多药炕药性 结肠癌 肿瘤 LoVo/5-FU 

分 类 号：R735.35[医药卫生—肿瘤]