慢性氟中毒对小鼠切牙胚内釉上皮TGF-beta1表达的影响  

Influence of chronic fluorine poisoning on the expression of TGF-beta1 in inner enamel epithelium of mouse incisor

作　　者：王大勇[1] 侯铁舟[1] 王强[1] 姚洪博[1] 赵涯东[1] 梁俊[1] 

机构地区：[1]西安交通大学口腔医学院,西安710004

出　　处：《陕西医学杂志》2004年第11期963-964,979,共3页Shaanxi Medical Journal

基　　金：陕西省科技攻关基金资助项目 (编号 :2 0 0 1 K1 0 -G9);西安交通大学重点科研培植项目 (编号 :P2 0 98)

摘　　要：目的 :观察慢性氟中毒对小鼠切牙胚内釉上皮表达 TGF- beta1的影响 ,探讨TGF- beta1在氟牙症发生机制中的作用。方法 :给小鼠分别喂以含 0、5 0、1 0 0 ppm Na F的饮水 ,6周后免疫组化观察结果。结果 :图像分析显示加氟组 TGF- beta1的表达水平低于空白组。结论 :氟可能通过抑制 TGF- beta1的表达干扰内釉上皮的分化和基质分泌 ,导致氟牙症的发生。Objective:To investigate the expression and distribution of TGF-beta1 protein in inner enamel epithelium of dental germ of mouse incisor afflicted with dental fluorosis. Methods:24 mice were divided into three groups and were fed with water of different sodium fluoride densities-0ppm,50ppm and 100ppm respectively.The incisors of those mice were extracted for immunohistochemical staining six weeks later. Results:Grey analysis shows that the expression levels of TGF-beta1 in 50ppm and 25ppm group were lower than that of 0ppm group.Conclusion: It's possible that by reducing the expression level of TGF-beta1 in inner enamel epithelium of dental germ of mouse incisor fluoride disturbs the proliferation of inner enamel epithelium cells.

关 键 词：慢性氟中毒 小鼠 牙胚 TGF-betal 表达 免疫组化 

分 类 号：R780.2[医药卫生—口腔医学]