p16基因转染对胰腺癌JF305细胞的抑制作用  

Suppressive effects of p16 gene on growth of pancreatic carcinoma cell line JF305

作　　者：马红兵[1] 胡海涛[1] 王西京[2] 夏辉[3] 李铮[3] 马洁[3] 

机构地区：[1]西安交通大学医学院 [2]西安交通大学第二医院肿瘤科,西安710004 [3]中国医学科学院肿瘤研究所分子肿瘤学国家重点实验室

出　　处：《陕西医学杂志》2004年第11期965-966,984,共3页Shaanxi Medical Journal

基　　金：陕西省科技攻关资助课题 (No.2 0 0 2 K1 0 -G3 ) ;西安交通大学第二医院科研基金资助课题 (No.2 0 0 1 YJ-0 4)

摘　　要：目的 :探讨抑癌基因 p1 6对胰腺癌 JF30 5细胞的抑制作用。方法 :以L ipofectamine TM2 0 0 0脂质体介导 p1 6基因转染胰腺癌 JF30 5细胞 ,MTT法测定 OD值 ,计算细胞生长抑制率 ,描绘 p1 6基因转染后 JF30 5细胞增殖曲线。结果 :p1 6基因转染对JF30 5细胞具有明显抑制作用 ( t=4.0 6,P<0 .0 1 ) ,细胞抑制率为 2 6.85 % ,细胞增殖曲线较 JF30 5细胞明显降低。结论 :p1 6基因转染对胰腺癌 JF30 5细胞具有明显抑制作用。Objective:To investigate the suppressive effects of p16 gene on the growth of pancreatic carcinoma cell line JF305. Methods:LipofectamineTM2000 liposome mediated p16 gene was transfected into pancreatic carcinoma cells JF305. MTT was used to detect the optical density value of the transfected cells and the growth curve of these cells was obtained. Results:The growth of p16 gene transfected cells was significantly inhibited than that of control group. The suppression rate of pancreatic carcinoma cell line JF305 with p16 transfection was 26.85%. The growth curve of this group was singnificantly lower than that of control group. Conclusion:The growth of pancreatic carcinoma cell line JF305 can be significantly suppressed by p16 gene transfection.

关 键 词：P16 基因转染 胰腺癌 JF305细胞 抑制作用 

分 类 号：R735.9[医药卫生—肿瘤]