Effects of selenium on peripheral blood mononuclear cell membrane fluidity,interleukin-2 production and interleukin-2 receptor expression in patients with chronic hepatitis  

作　　者：Shui-XiangHe BingWu Xin-MingChang Hong-XiaLi WenQiao 

机构地区：[1]DepartmentofGastroenterolgy,FirstHospitalofXi'anJiaotongUniversity,Xi'an710061,ShaanxiProvince,China [2]EditorialDepartmentofJournalofXi'anJiaotongUniversity,Xi'an710061,ShaanxiProvince,China [4]DepartmentofGastroenterolgy,FirstHospitalofXi'anJiaotongUniversity,Xi'an710061,ShaanxiProvince,China

出　　处：《World Journal of Gastroenterology》2004年第23期3531-3533,共3页世界胃肠病学杂志（英文版）

摘　　要：AIM To study the effect of selenium on peripheral blood mononuclear cell (PBMC) membrane fluidity and immune function in patients with chronic hepatitis.METHODS: PBMCs were pretreated with selenium (1.156x 10.7 mol/L) for 6 h in vitro or extracted directly from patients after administration of selenium-yeast continuously for 8-12 wk (200 pg/d), and then exposed to Con-A for 48 h. The membrane fluidity, interleukin-2 (IL-2) production and interleukin-2 receptor (IL-2R) expression in PBMCs and malondialdehyde (MDA) concentration in medium and lipid peroxide (LPO) in plasma were determined.RESULTS: The PBMC membrane fluidity, IL-2 production and IL-2R expression in patients with chronic hepatitis were significantly lower than those in healthy blood donators (particle adhesive degree R, 0.17±0.01 vs 0.14±0.01,P<0.01; IL-2, 40.26±9.55 vs72.96±11.36, P<0.01, IL-2R,31.05±5.09 vs 60.58±10.56, P<0.01), and the MDA concentration in medium in patients with chronic hepatitis was significantly higher than that in healthy blood donators (1.44±0.08 vs0.93±0.08, P<0.01). Both in vitro and in vivo administration of selenium could reverse the above parameters.CONCLUSION: Supplement of selenium can suppress lipid peroxidation, and improve PBMC membrane fluidity and immune function in patients with chronic hepatitis.AIM:To study the effect of selenium on peripheral blood mononuclear cell(PBMC)membrane fluidity and immune function in patients with chronic hepatitis. METHODS:PBMCs were pretreated with selenium (1.156×10^(-7)mol/L)for 6 h in v/boor extracted directly from patients after administration of selenium-yeast continuously for 8-12 wk(200 μg/d),and then exposed to Con-A for 48 h.The membrane fluidity,interleukJn-2(IL-2)production and interleukin-2 receptor(IL-2R)expression in PBMCs and malondialdehyde(MDA)concentration in medium and lipid peroxide(LPO)in plasma were determined. RESULTS:The PBMC membrane fluidity,IL-2 production and IL-2R expression in patients with chronic hepatitis were significantly lower than those in healthy blood donators (particle adhesive degree R,0.17±0.01 vs 0.14±0.01, P<0.01;IL-2,40.26+9.55 vs72.96±11.36,P<0.01;IL-2R, 31.05±5.09 vs 60.58±10.56,P<0.01),and the MDA concentration in medium in patients with chronic hepatitis was significantly higher than that in healthy blood donators (1.44±0.08 vs0.93±0.08,P<0.01).Both in vib-o and in vivo administration of selenium could reverse the above parameters. CONCLUSION:Supplement of selenium can suppress lipid peroxidation,and improve PBMC membrane fluidity and immune function in patients with chronic hepatitis.

关 键 词：外周血 单核细胞 膜流动性 白细胞介素-2 受体表达 慢性肝炎 肝脏 

分 类 号：R575.1[医药卫生—消化系统]