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作 者:杜建时[1] 赵丹[2] 狄茜[2] 杨宝学[3] 赵雪俭[2]
机构地区:[1]吉林大学中日联谊医院血管外科 [2]吉林大学基础医学院病理生理学教研室 [3]美国加利福尼亚大学旧金山医学院CA 94122
出 处:《吉林大学学报(医学版)》2004年第6期893-896,共4页Journal of Jilin University:Medicine Edition
基 金:吉林省科技厅资助课题 (2 0 0 30 5 37- 2 )
摘 要:目的 :探讨水通道蛋白 7和 8(aquaporin 7,AQP7;aquaporin 8,AQP8)的表达与睾丸生精功能的关系 ,以及人参二醇皂苷 (PDS)对睾丸水通道蛋白表达的影响。方法 :4、 12周龄 Wistar大鼠分为盐水对照组(Con组 ) (n=6 )和 PDS腹腔注射组 (PDS组 ) (n=6 )。PDS组每日给予 PDS腹腔注射 (2 5 mg· kg- 1 · d- 1 ,1m L ) ,Con组注射等体积生理盐水 ,给药 2周。采用半定量 RT- PCR和 Western blotting比较睾丸 AQP7与 AQP8的m RNA和蛋白质表达水平。结果 :随增龄睾丸 AQP7和 AQP8m RNA和蛋白表达明显增高 ;4周龄大鼠 PDS腹腔注射 2周后睾丸 AQP7和 AQP8表达增强 ,PDS对 4周龄大鼠睾丸 AQP7蛋白质表达有明显的上调作用 ,12周龄大鼠接受 14 d PDS腹腔注射后其 AQP8蛋白质表达水平略有下降。结论 :睾丸 AQP7和 AQP8的表达随性成熟明显增高 ;PDS对睾丸水通道表达有明显的调节作用。Objective To investigate the relationship between the expressions of aquaporin 7 (AQP7) and aquaporin 8(AQP8) and the spermatogenesis of rat testis, and the effects of panaxadiol saponins(PDS) on expressions of AQP7 and AQP8. Methods Wistar rats of 4 and 12 weeks old were divided into saline control (Con group) and PDS groups. The rats in PDS group were injected with PDS in abdominal cavity (25 mg·kg -1 ·d -1 ,1 mL) every day, and saline was used in Con group with equal volume for 2 weeks. Semi-quantitative RT-PCR and Western blotting were employed to detect the mRNA and protein expression levels of AQP7 and AQP8. Results Expression levels of AQP7 and AQP8 were elevated when the age of rats increased; two weeks after injection of PDS, the expressions of AQP7 and AQP8 were upregulated in 4 weeks old rats, but the expressions of AQP7 and AQP8 in 12 weeks old rats were inhibited. Conclusion The expression levels of AQP7 and AQP8 are increased significantly with the sexual maturation; PDS can regulate the expressions of AQPs in testis.
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