人端粒重复序列结合因子在急性白血病细胞中的表达及与端粒酶活性的关系  

Expression of human telomere repeat binding factor1 (TRF1) in acute leukemia cells and its correlation with telomerase activities

作　　者：孙洁[1] 来晓瑜[1] 朱园园[1] 蓝建平[1] 汤立旦[1] 李静远[1] 余建[1] 谭亚敏[1] 林茂芳[1] 黄河[1] 

机构地区：[1]浙江大学医学院附属第一医院,浙江杭州310003

出　　处：《浙江大学学报（医学版）》2004年第6期491-495,共5页Journal of Zhejiang University(Medical Sciences)

基　　金：国家重点基础研究发展规划项目 (2 0 0 2 CB71370 0 );国家自然科学基金资助项目 (39870 339);浙江省自然科学基金资助项目 (39914 1)

摘　　要：目的 :研究人端粒重复序列结合因子 (TRF1)在 30例急性白血病细胞中的表达 ,了解 TRF1表达与急性白血病分型的关系 ,以及与端粒酶活性的关系。方法 :用荧光定量 RT- PCR定量检测 30例急性白血病细胞中TRF1m RNA表达情况 ,同时检测 h TERT m RNA的表达 ,并分析两者之间的相关性。结果 :TRF1在急性非淋巴细胞性白血病 (ANLL)细胞中的表达 0 .0 12 6 (0 .0 12 7～ 0 .0 5 46 )明显低于正常人骨髓单个核细胞中的表达 0 .0 4 5 7(0 .0 0 839～ 0 .2 6 2 ) (P<0 .0 0 1) ,但在急性淋巴细胞性白血病 (ALL)细胞中的表达 0 .0 74 5 (1.92× 10 -6～ 0 .193)与正常人骨髓单个核细胞比较差异无显著性 ,且在 ANL L细胞中的表达明显低于 ALL细胞中的表达 (P=0 .0 0 1)。TRF1和 h TERT在急性白血病细胞和正常人骨髓单个核细胞中的表达无显著相关性 (r=- 0 .173,P=0 .2 0 7)。结论 :TRF1m RNA在 ANLL细胞中表达明显降低 ,而在 ALL细胞中表达无显著改变 ,提示 ANLL细胞中 TRF1可能通过调控端粒长度对端粒酶起间接负调控作用。Objective: To study the expression of human telomere repeat binding factor1 (TRF1) to investigate the correlation of telomerase activity with acute leukemia. Methods: Leukemic cells were collected from 30 cases of acute leukemia. Realtime quantitative PCR with fluorescence probe hybridization was used to measure expression of TRF1 and hTERT mRNA in leukemic cells. Results: TRF1 mRNA expression was 0 0126 (0 0127～0 0546) in acute non lymphocytic leukemia (ANLL), which was lower than that in normal mononuclear cells [0 0457(0 00839～0 262), P <0 001], but its expression in acute lymphoblastic leukemia (ALL) cells [0 0745(1 92×10 -6 ～0 193) ] had no significant difference compared with that in normal mononuclear cells.TRF1 expression in ANLL cells was significantly lower than that in ALL cells( P =0 001).The expressions of TRF1 mRNA in AL cells and normal mononuclear cells had no significant correlation with expression of hTERT mRNA( r =-0 173, P =0 207). Conclusion: The expression of TRF1 is lower in ANLL cells,which indicates TRF1 may have some effect on telomerase activity by regulating telomere length in ANLL cells. [

关 键 词：端粒 端粒酶 人端粒重复序列结合因子 急性白血病 表达 

分 类 号：R733.71[医药卫生—肿瘤]